ArticleScientific reports2015
Regulation of DNA phosphorothioate modification in Salmonella enterica by DndB.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Biology of host-dependent restriction-modification in prokaryotes.EcoSal Plus · 2025Review
- Versatile NTP recognition and domain fusions expand the functional repertoire of the ParB-CTPase fold beyond chromosome segregation.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- Biosynthesis and function of 7-deazaguanine derivatives in bacteria and phages.Microbiology and molecular biology reviews : MMBR · 2024Review
- Reappraisal of the DNA phosphorothioate modification machinery: uncovering neglected functional modalities and identification of new counter-invader defense systems.Nucleic acids research · 2024Article
- The DNA Phosphorothioation Restriction-Modification System Influences the Antimicrobial Resistance of Pathogenic Bacteria.Microbiology spectrum · 2023Article
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- Nanopore Sequencing for Detection and Characterization of Phosphorothioate Modifications in Native DNA Sequences.Frontiers in microbiology · 2022Article
- Rapid identification of magnesium ascorbyl phosphate utilizing phosphatase through a chromogenic change-coupled activity assay.Applied microbiology and biotechnology · 2021Article
- Comprehensive classification of ABC ATPases and their functional radiation in nucleoprotein dynamics and biological conflict systems.Nucleic acids research · 2020Review
- Epigenetic competition reveals density-dependent regulation and target site plasticity of phosphorothioate epigenetics in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Structural Analysis of an l-Cysteine Desulfurase from an Ssp DNA Phosphorothioation System.mBio · 2020Article
- A new type of DNA phosphorothioation-based antiviral system in archaea.Nature communications · 2019Article
- DNA phosphorothioate modification-a new multi-functional epigenetic system in bacteria.FEMS microbiology reviews · 2019Review
- Identification of the minimal bacterial 2'-deoxy-7-amido-7-deazaguanine synthesis machinery.Molecular microbiology · 2018Article
- Identification of a functional toxin-antitoxin system located in the genomic island PYG1 of piezophilic hyperthermophilic archaeon Pyrococcus yayanosii.Extremophiles : life under extreme conditions · 2018Article
- Occurrence, evolution, and functions of DNA phosphorothioate epigenetics in bacteria.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Regulation ofFrontiers in microbiology · 2018Article
- Evolutionary Genomics of Defense Systems in Archaea and Bacteria.Annual review of microbiology · 2017Review
- Deazaguanine derivatives, examples of crosstalk between RNA and DNA modification pathways.RNA biology · 2017Review
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
DNA phosphorothioate (PT) modification, in which the non-bridging oxygen of the sugar-phosphate backbone is substituted by sulfur, occurs naturally in diverse bacteria and archaea and is regulated by the DndABCDE proteins. DndABCDE and the restriction cognate DndFGHI constitute a new type of defense system that prevents the invasion of foreign DNA in Salmonella enterica serovar Cerro 87. GAAC/GTTC consensus contexts across genomes were found to possess partial PT modifications even in the presence of restriction activity, indicating the regulation of PT. The abundance of PT in cells must be controlled to suit cellular activities. However, the regulatory mechanism of PT modification has not been characterized. The result here indicated that genomic PT modification in S. enterica is controlled by the transcriptional regulator DndB, which binds to two regions in the promoter, each possessing a 5'-TACGN(10)CGTA-3' palindromic motif, to regulate the transcription of dndCDE and its own gene. Site-directed mutagenesis showed that the Cys29 residue of DndB plays a key role in its DNA-binding activity or conformation. Proteomic analysis identified changes to a number of cellular proteins upon up-regulation and loss of PT. Considering the genetic conservation of dnd operons, regulation of PT by DndB might be widespread in diverse organisms.
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