Evidence map›Paper›PMID 26189505›Full record

ArticleLasers in surgery and medicine2015

Enzymatically activated near infrared nanoprobes based on amphiphilic block copolymers for optical detection of cancer.

Tuğba Özel, Sean White, Elaine Nguyen, Austin Moy, Nicholas Brenes, Bernard Choi, Tania Betancourt

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Lasers in surgery and medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.1field-weighted citation impact, top 24% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 13 citations in OpenAlex.

  1. Review
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  4. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 5 institutions in 1 country.

Tuğba ÖzelMaterials Science, Engineering, and Commercialization Program, Texas State University, San Marcos, Texas 78666.
Sean WhiteDepartment of Biomedical Engineering, Beckman Laser Institute, University of California, Irvine, California 92697.
Elaine NguyenDepartment of Biomedical Engineering, Beckman Laser Institute, University of California, Irvine, California 92697.
Austin MoyDepartment of Biomedical Engineering, Beckman Laser Institute, University of California, Irvine, California 92697.
Nicholas BrenesThe University of Texas at Austin, Austin, Texas 78712.
Bernard ChoiDepartment of Biomedical Engineering, Beckman Laser Institute, University of California, Irvine, California 92697.
Tania BetancourtMaterials Science, Engineering, and Commercialization Program, Texas State University, San Marcos, Texas 78666.
University of California, Irvine · USBeckman Laser Institute and Medical Clinic · USInnoSense (United States) · USTexas State University · USThe University of Texas at Austin · US

Funding

Virtual Photonocs TechnologiesP41EB015890 · NIBIB · UNIVERSITY OF CALIFORNIA-IRVINE · PI DURKIN, ANTHONY · 2012 to 2017
$7.0M
Novel Optical Treatment Approach for Vascular BirthmarksR01HD065536 · NICHD · UNIVERSITY OF CALIFORNIA-IRVINE · PI CHOI, BERNARD, KELLY, KRISTEN M · 2010 to 2014
$1.4M
Enzyme-activated Nanoparticles as Contrast Agents for Optical Detection of CancerR43CA144129 · NCI · INNOSENSE, LLC · PI BETANCOURT, TANIA · 2010 to 2010
$184k
NCI NIH HHS R43 CA144129NIBIB NIH HHS P41 EB015890NICHD NIH HHS R01 HD065536
6 · The paper itself

Abstract

BACKGROUND AND

objectiveNanotechnology offers the possibility of creating multi-functional structures that can provide solutions for biomedical problems. The nanoprobes herein described are an example of such structures, where nano-scaled particles have been designed to provide high specificity and contrast potential for optical detection of cancer. Specifically, enzymatically activated fluorescent nanoprobes (EANPs) were synthesized as cancer-specific contrast agents for optical imaging. STUDY DESIGN/MATERIALS AND

methodsEANPs were prepared by nanoprecipitation of blends of poly(lactic acid)-b-poly(ethylene glycol) and poly(lactic-co-glycolic acid)-b-poly(l-lysine). The lysine moieties were then covalently decorated with the near infrared (NIR) fluorescent molecule AlexaFluor-750 (AF750). Close proximity of the fluorescent molecules to each other resulted in fluorescence quenching, which was reversed by enzymatically mediated cleavage of poly(l-lysine) chains. EANPs were characterized by dynamic light scattering and electron microscopy. Enzymatic development of fluorescence was studied in vitro by fluorescence spectroscopy. Biocompatibility and contrast potential of EANPs were studied in cancerous and noncancerous cells. The potential of the nanoprobes as contrast agents for NIR fluorescence imaging was studied in tissue phantoms.

resultsSpherical EANPs of ∼100 nm were synthesized via nanoprecipitation of polymer blends. Fluorescence activation of EANPs by treatment with a model protease was demonstrated with up to 15-fold optical signal enhancement within 120 minutes. Studies with MDA-MB-231 breast cancer cells demonstrated the cytocompatibility of EANPs, as well as enhanced fluorescence associated with enzymatic activation. Imaging studies in tissue phantoms confirmed the ability of a simple imaging system based on a laser source and CCD camera to image dilute suspensions of the nanoprobe at depths of up to 4 mm, as well as up to a 13-fold signal-to-background ratio for enzymatically activated EANPs compared to un-activated EANPs at the same concentration.

conclusionNanoprecipitation of copolymer blends containing poly(l-lysine) was utilized as a method for preparation of highly functional nanoprobes with high potential as contrast agents for fluorescence based imaging of cancer. Lasers Surg. Med. 47:579-594, 2015. © 2015 Wiley Periodicals, Inc.

Indexed as

biodegradable polymersenzymatically-responsive polymersnanomedicinenanoparticlesnear infrared fluorescent contrast agentsnear infrared optical imagingoptical contrast agentsPLA-PEGPLGAprotease activated probes

Identifiers

PMID26189505
PMCPMC4716005
OpenAlexW1905198696

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.