Evidence map›Paper›PMID 26188545›Full record

ArticleFEBS letters2015

Potential regulatory phosphorylation sites in a Medicago truncatula plasma membrane proton pump implicated during early symbiotic signaling in roots.

Thao T Nguyen, Jeremy D Volkening, Christopher M Rose, Muthusubramanian Venkateshwaran, Michael S Westphall, Joshua J Coon, Jean-Michel Ané, Michael R Sussman

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in FEBS letters, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
3.6field-weighted citation impact, top 8% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed, 12 citations in OpenAlex.

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  4. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 3 institutions in 1 country.

Thao T NguyenDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States.
Jeremy D VolkeningDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States.
Christopher M RoseDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States.
Muthusubramanian VenkateshwaranDepartment of Agronomy, University of Wisconsin-Madison, Madison, WI 53706, United States; School of Agriculture, University of Wisconsin-Platteville, Platteville, WI 53818, United States.
Michael S WestphallDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States.
Joshua J CoonDepartment of Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Genome Center of Wisconsin, University of Wisconsin-Madison, Madison, WI 53706, United States; Department of Biomolecular Chemistry, University of Wisconsin-Madison, Madison, WI 53706, United States.
Jean-Michel AnéDepartment of Agronomy, University of Wisconsin-Madison, Madison, WI 53706, United States.
Michael R SussmanDepartment of Biochemistry, University of Wisconsin-Madison, Madison, WI 53706, United States; Biotechnology Center, University of Wisconsin-Madison, Madison, WI 53706, United States. Electronic address: msussman@wisc.edu.
University of Wisconsin–Madison · USEnergy Center of Wisconsin · USUniversity of Wisconsin–Platteville · US

Funding

Chemistry-Biology Interface Training ProgramT32GM008505 · NIGMS · UNIVERSITY OF WISCONSIN-MADISON · PI BLACKWELL, HELEN E. · 1993 to 2023
$9.8M
NIGMS NIH HHS T32 GM008505NIGMS NIH HHS T32GM008505
6 · The paper itself

Abstract

In plants and fungi the plasma membrane proton pump generates a large proton-motive force that performs essential functions in many processes, including solute transport and the control of cell elongation. Previous studies in yeast and higher plants have indicated that phosphorylation of an auto-inhibitory domain is involved in regulating pump activity. In this report we examine the Medicago truncatula plasma membrane proton pump gene family, and in particular MtAHA5. Yeast complementation assays with phosphomimetic mutations at six candidate sites support a phosphoregulatory role for two residues, suggesting a molecular model to explain early Nod factor-induced changes in the plasma membrane proton-motive force of legume root cells.

Indexed as

Amino Acid SequenceBinding SitesBlotting, WesternCell MembraneCluster AnalysisGene Expression Regulation, EnzymologicGenetic Complementation TestHost-Pathogen InteractionsMedicago truncatulaMolecular Sequence DataMultigene FamilyMutationPhosphorylationPhylogenyPlant ProteinsPlant RootsPlant ProteinsProton-Translocating ATPasesSerineThreonineMedicago truncatulaNod factorPhosphoproteomicsPhosphorylationPM H(+)-ATPaseProton pump

Identifiers

PMID26188545
PMCPMC5991090
OpenAlexW1650607029

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.