ArticlePlant molecular biology2015
Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 155 papers.
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Who cites it
155 citing papers in PubMed, 306 citations in OpenAlex.
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- Identification of Gene Regulatory Network for Nitrogen-Promoted Tiller Regrowth in Perennial Rice.Rice (New York, N.Y.) · 2026Article
- A Rapid Hairy Root-Based Platform for CRISPR/Cas Optimization and Guide RNA Validation in Lettuce.Plants (Basel, Switzerland) · 2026Article
- Functional characterization of transporter genes in rice node at single-cell resolution through multi-omics technologies.Genome biology · 2026Article
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- OsFPN1 functions as an essential metal transporter for Fe homeostasis and Co/Ni tolerance in rice.Plant molecular biology · 2026Article
- Artificial Mutations in the Nuclear Gene Encoding Mitochondrial RNA Polymerase Restore Pollen Fertility in Cytoplasmic Male Sterile Tomato.Plant biotechnology journal · 2026Article
- Knockout of a single aquaporin, OsPIP2;4, decreases root water permeability in rice.Journal of plant research · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- CRISPR/Cas9-mediated knockout ofFrontiers in genome editing · 2026Article
- Florigen and cytokinin signaling antagonistically regulate FLOWERING LOCUS T-LIKE1 to drive a florigen relay that facilitates inflorescence development in rice.Science advances · 2025Article
- A node-localized efflux transporter for loading iron to developing tissues in rice.Nature communications · 2025Article
- The rice BRITTLE CULM 4 gene encodes a membrane protein affecting cellulose synthesis in the secondary cell wall.Plant & cell physiology · 2025Article
- CRISPR/Cas-mediated genome editing: playing a versatile role in mitigating the challenges of sustainable rice improvement.3 Biotech · 2025Review
- Chelation-based iron uptake mitigates the effects of prolonged high-temperature stress in cool-season grasses.Nature communications · 2025Article
- A 65-kb deletion survey identifies a distalProceedings of the National Academy of Sciences of the United States of America · 2025Article
95 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The CRISPR/Cas9 system is an efficient tool used for genome editing in a variety of organisms. Despite several recent reports of successful targeted mutagenesis using the CRISPR/Cas9 system in plants, in each case the target gene of interest, the Cas9 expression system and guide-RNA (gRNA) used, and the tissues used for transformation and subsequent mutagenesis differed, hence the reported frequencies of targeted mutagenesis cannot be compared directly. Here, we evaluated mutation frequency in rice using different Cas9 and/or gRNA expression cassettes under standardized experimental conditions. We introduced Cas9 and gRNA expression cassettes separately or sequentially into rice calli, and assessed the frequency of mutagenesis at the same endogenous targeted sequences. Mutation frequencies differed significantly depending on the Cas9 expression cassette used. In addition, a gRNA driven by the OsU6 promoter was superior to one driven by the OsU3 promoter. Using an all-in-one expression vector harboring the best combined Cas9/gRNA expression cassette resulted in a much improved frequency of targeted mutagenesis in rice calli, and bi-allelic mutant plants were produced in the T0 generation. The approach presented here could be adapted to optimize the construction of Cas9/gRNA cassettes for genome editing in a variety of plants.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.