Evidence map›Paper›PMID 26188381›Full record

ArticleExperimental neurology2015

Progesterone protects endothelial cells after cerebrovascular occlusion by decreasing MCP-1- and CXCL1-mediated macrophage infiltration.

Ebony Washington Remus, Iqbal Sayeed, Soonmi Won, Alicia N Lyle, Donald G Stein

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Experimental neurology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.

0numbers the graph read from it
0cells of the map it votes in
15citing papers in PubMed
2.8field-weighted citation impact, top 10% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

15 citing papers in PubMed, 32 citations in OpenAlex.

  1. Review
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  4. Stem Cell- and Cell-Based Therapies for Ischemic Stroke.Bioengineering (Basel, Switzerland) · 2022
    Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Ebony Washington RemusDepartment of Emergency Medicine Brain Research Laboratory, Emory University, Atlanta, GA, USA.
Iqbal SayeedDepartment of Emergency Medicine Brain Research Laboratory, Emory University, Atlanta, GA, USA.
Soonmi WonDepartment of Emergency Medicine Brain Research Laboratory, Emory University, Atlanta, GA, USA.
Alicia N LyleDepartment of Cardiology, Emory University Atlanta, GA, USA.
Donald G SteinDepartment of Emergency Medicine Brain Research Laboratory, Emory University, Atlanta, GA, USA. Electronic address: dstei04@emory.edu.
Emory University · US

Funding

Progesterone in the Treatment of ischemic StrokeU01NS062676 · NINDS · EMORY UNIVERSITY · PI STEIN, DONALD G. · 2010 to 2012
$2.4M
NINDS NIH HHS NIH UO1 NS062676NINDS NIH HHS U01 NS062676
6 · The paper itself

Abstract

The neuroprotective effects of progesterone after ischemic stroke have been established, but the role of progesterone in promoting cerebrovascular repair remains under-explored. Male Sprague-Dawley rats underwent transient middle cerebral artery occlusion (tMCAO) for 90 min followed by reperfusion for 3 days. Progesterone (8 mg/kg/day) was administered intraperitoneally at 1h after initial occlusion followed by subcutaneous injections at 6, 24 and 48 h post-occlusion. Rats were euthanized after 72 h and brain endothelial cell density and macrophage infiltration were evaluated within the cerebral cortex. We also assessed progesterone's ability to induce macrophage migration toward hypoxic/reoxygenated cultured endothelial cells. We found that progesterone treatment post-tMCAO protects ischemic endothelial cells from macrophage infiltration. We further demonstrate that infiltration of monocytes/macrophages can be induced by potent chemotactic factors such as monocyte chemoattractant protein-1 (MCP-1) and the chemokine ligand 1 (CXCL1), secreted by hypoxic/reoxygenated endothelial cells. Progesterone blunts secretion of MCP-1 and CXCL1 from endothelial cells after hypoxia/reoxygenation injury and decreases leukocyte infiltration. The treatment protects ischemic endothelial cells from macrophage infiltration and thus preserves vascularization after ischemic injury.

Indexed as

Infarction, Middle Cerebral ArteryAnimalsAntigens, CDAntigens, Differentiation, MyelomonocyticCD68 MoleculeCell HypoxiaCell MovementCells, CulturedChemokine CCL2Chemokine CXCL1CytokinesDisease Models, AnimalEndothelial CellsGene Expression RegulationKi-67 AntigenMacrophagesAntigens, CDAntigens, Differentiation, MyelomonocyticCcl2 protein, ratCD68 antigen, humanCD68 MoleculeChemokine CCL2Chemokine CXCL1Cxcl1 protein, ratCytokinesKi-67 AntigenProgesteroneProgestinsBrain ischemiaCerebrovascular repairEndothelial cellsMacrophage infiltrationProgesterone

Identifiers

PMID26188381
PMCPMC4586408
OpenAlexW1918411450

What OpenQuestion holds

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LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.