ArticleJournal of experimental botany2015
Three genes encoding AOP2, a protein involved in aliphatic glucosinolate biosynthesis, are differentially expressed in Brassica rapa.
Article in Journal of experimental botany, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 36 citations in OpenAlex.
- Bivalent Histone Modifications Orchestrate Temporal Regulation of Glucosinolate Biosynthesis During Wound-Induced Stress Responses in Arabidopsis.Plant, cell & environment · 2026Article
- A naturally occurring promoter variation ofFrontiers in plant science · 2025Article
- Metabolomic and Transcriptomic Profiles in DiverseJournal of agricultural and food chemistry · 2024Article
- Chromosome-scale reference genome of broccoli (Horticulture research · 2024Article
- Developing multifunctional crops by engineering Brassicaceae glucosinolate pathways.Plant communications · 2023Review
- Glucosinolates and Biotic Stress Tolerance in Brassicaceae with Emphasis on Cabbage: A Review.Biochemical genetics · 2023Review
- Glucosinolate Biosynthetic Genes of Cabbage: Genome-Wide Identification, Evolution, and Expression Analysis.Genes · 2023Article
- Identification andFrontiers in plant science · 2023Article
- Transcriptome analysis of genes related to glucoraphanin and sulforaphane synthesis in methyl jasmonate treated broccoli (Brassica oleracea var. italica) hairy roots.Journal of plant research · 2022Article
- Mapping and Validation ofInternational journal of molecular sciences · 2022Article
- Transcriptome analysis of melatonin regulating the transformation of glucoraphanin to sulforaphane in broccoli hairy roots.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Article
- Transcriptome and QTL mapping analyses of major QTL genes controlling glucosinolate contents in vegetable- and oilseed-typeFrontiers in plant science · 2022Article
- Transcriptomics analysis of genes induced by melatonin related to glucosinolates synthesis in broccoli hairy roots.Plant signaling & behavior · 2021Article
- Influence of Genotype on High Glucosinolate Synthesis Lines ofInternational journal of molecular sciences · 2021Article
- Divergence of three BRX homoeologs in Brassica rapa and its effect on leaf morphology.Horticulture research · 2021Article
- Glucosinolate Profile and Glucosinolate Biosynthesis and Breakdown Gene Expression Manifested by Black Rot Disease Infection in Cabbage.Plants (Basel, Switzerland) · 2020Article
- Differential expression of major genes involved in the biosynthesis of aliphatic glucosinolates in intergeneric Baemoochae (Brassicaceae) and its parents during development.Plant molecular biology · 2020Article
- Overexpression of the MYB29 transcription factor affects aliphatic glucosinolate synthesis in Brassica oleracea.Plant molecular biology · 2019Article
- Comparative transcriptome analyses revealed different heat stress responses in high- and low-GS Brassica alboglabra sprouts.BMC genomics · 2019Article
- A naturally occurring variation in theHorticulture research · 2018Article
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Authors and funding
6 authors at 2 institutions in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
The glucosinolate biosynthetic gene AOP2 encodes an enzyme that plays a crucial role in catalysing the conversion of beneficial glucosinolates into anti-nutritional ones. In Brassica rapa, three copies of BrAOP2 have been identified, but their function in establishing the glucosinolate content of B. rapa is poorly understood. Here, we used phylogenetic and gene structure analyses to show that BrAOP2 proteins have evolved via a duplication process retaining two highly conserved domains at the N-terminal and C-terminal regions, while the middle part has experienced structural divergence. Heterologous expression and in vitro enzyme assays and Arabidopsis mutant complementation studies showed that all three BrAOP2 genes encode functional BrAOP2 proteins that convert the precursor methylsulfinyl alkyl glucosinolate to the alkenyl form. Site-directed mutagenesis showed that His356, Asp310, and Arg376 residues are required for the catalytic activity of one of the BrAOP2 proteins (BrAOP2.1). Promoter-β-glucuronidase lines revealed that the BrAOP2.3 gene displayed an overlapping but distinct tissue- and cell-specific expression profile compared with that of the BrAOP2.1 and BrAOP2.2 genes. Quantitative real-time reverse transcription-PCR assays demonstrated that BrAOP2.1 showed a slightly different pattern of expression in below-ground tissue at the seedling stage and in the silique at the reproductive stage compared with BrAOP2.2 and BrAOP2.3 genes in B. rapa. Taken together, our results revealed that all three BrAOP2 paralogues are active in B. rapa but have functionally diverged.
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