ArticleApplied and environmental microbiology2015
Quantification of Azospirillum brasilense FP2 Bacteria in Wheat Roots by Strain-Specific Quantitative PCR.
Article in Applied and environmental microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed.
- Real-Time PCR (qtPCR) to Discover the Fate of Plant Growth-Promoting Rhizobacteria (PGPR) in Agricultural Soils.Microorganisms · 2024Article
- Long-term detection of Hartmannibacter diazotrophicus on winter wheat and spring barley roots under field conditions revealed positive correlations on yield parameters with the bacterium abundance.FEMS microbiology ecology · 2024Article
- Designing and validation of specific primers for the quantitative detection of bacteria in sugarcane inoculant.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2023Article
- Optimization of Molecular Methods for Detecting Duckweed-Associated Bacteria.Plants (Basel, Switzerland) · 2023Article
- Meta-omics integration approach reveals the effect of soil native microbiome diversity in the performance of inoculantFrontiers in plant science · 2023Article
- Pangenomes-identified singletons for designing specific primers to identify bacterial strains in a plant growth-promoting consortium.Molecular biology reports · 2022Article
- Inoculation of Herbaspirillum seropedicae strain SmR1 increases biomass in maize roots DKB 390 variety in the early stages of plant development.Archives of microbiology · 2022Article
- Biofertilizer microorganisms accompanying pathogenic attributes: a potential threat.Physiology and molecular biology of plants : an international journal of functional plant biology · 2022Review
- Effectiveness of Plant Beneficial Microbes: Overview of the Methodological Approaches for the Assessment of Root Colonization and Persistence.Frontiers in plant science · 2020Review
- Two-step genomic sequence comparison strategy to design Trichoderma strain-specific primers for quantitative PCR.AMB Express · 2019Article
- Assessment of the structural and functional diversities of plant microbiota: Achievements and challenges - A review.Journal of advanced research · 2019Review
- Physiology and molecular biology of plants : an international journal of functional plant biology · 2019Article
- Application of Bacillus velezensis NJAU-Z9 Enhanced Plant Growth Associated with Efficient Rhizospheric Colonization Monitored by qPCR with Primers Designed from the Whole Genome Sequence.Current microbiology · 2018Article
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7 authors.
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Abstract
Azospirillum is a rhizobacterial genus containing plant growth-promoting species associated with different crops worldwide. Azospirillum brasilense strains exhibit a growth-promoting effect by means of phytohormone production and possibly by N2 fixation. However, one of the most important factors for achieving an increase in crop yield by plant growth-promoting rhizobacteria is the survival of the inoculant in the rhizosphere, which is not always achieved. The objective of this study was to develop quantitative PCR protocols for the strain-specific quantification of A. brasilense FP2. A novel approach was applied to identify strain-specific DNA sequences based on a comparison of the genomic sequences within the same species. The draft genome sequences of A. brasilense FP2 and Sp245 were aligned, and FP2-specific regions were filtered and checked for other possible matches in public databases. Strain-specific regions were then selected to design and evaluate strain-specific primer pairs. The primer pairs AzoR2.1, AzoR2.2, AzoR5.1, AzoR5.2, and AzoR5.3 were specific for the A. brasilense FP2 strain. These primer pairs were used to monitor quantitatively the population of A. brasilense in wheat roots under sterile and nonsterile growth conditions. In addition, coinoculations with other plant growth-promoting bacteria in wheat were performed under nonsterile conditions. The results showed that A. brasilense FP2 inoculated into wheat roots is highly competitive and achieves high cell numbers (∼10(7) CFU/g [fresh weight] of root) in the rhizosphere even under nonsterile conditions and when coinoculated with other rhizobacteria, maintaining the population at rather stable levels for at least up to 13 days after inoculation. The strategy used here can be applied to other organisms whose genome sequences are available.
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