ArticleMolecular human reproduction2015
Rescue of failed oocyte activation after ICSI in a mouse model of male factor infertility by recombinant phospholipase Cζ.
Article in Molecular human reproduction, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 61 citations in OpenAlex.
- A comprehensive review about sperm-oocyte interactions and key activation factors of fertilization in mammals.The Canadian journal of urology · 2026Review
- Phospholipase Cζ, the Molecular Spark of Fertilization and Male Infertility: Insights from Bench to Bedside.Medicina (Kaunas, Lithuania) · 2025Review
- Development of Recombinant PLC-Zeta Protein as a Therapeutic Intervention for the Clinical Treatment of Oocyte Activation Failure.Biomedicines · 2024Article
- Phospholipase C Zeta 1 (PLCZ1): The Function and Potential for Fertility Assessment and In Vitro Embryo Production in Cattle and Horses.Veterinary sciences · 2023Review
- The Therapeutic and Diagnostic Potential of Phospholipase C Zeta, Oocyte Activation, and Calcium in Treating Human Infertility.Pharmaceuticals (Basel, Switzerland) · 2023Review
- Early rescue oocyte activation at 5 h post-ICSI is a useful strategy for avoiding unexpected fertilization failure and low fertilization in ICSI cycles.Frontiers in endocrinology · 2023Observational
- Applications of advances in mRNA-based platforms as therapeutics and diagnostics in reproductive technologies.Frontiers in cell and developmental biology · 2023Review
- SPERM FACTORS AND EGG ACTIVATION: Phospholipase C zeta (PLCZ1) and the clinical diagnosis of oocyte activation deficiency.Reproduction (Cambridge, England) · 2022Review
- Oocyte activation deficiency and assisted oocyte activation: mechanisms, obstacles and prospects for clinical application.Human reproduction open · 2022Review
- Strategies to Identify Genetic Variants Causing Infertility.Trends in molecular medicine · 2021Review
- The role of ATP in the differential ability of Sr2+ to trigger Ca2+ oscillations in mouse and human eggs.Molecular human reproduction · 2021Article
- Sperm-oocyte interplay: an overview of spermatozoon's role in oocyte activation and current perspectives in diagnosis and fertility treatment.Cell & bioscience · 2021Review
- Modulators of calcium signalling at fertilization.Open biology · 2020Review
- Increasing associations between defects in phospholipase C zeta and conditions of male infertility: not just ICSI failure?Journal of assisted reproduction and genetics · 2020Review
- Male Infertility is a Women's Health Issue-Research and Clinical Evaluation of Male Infertility Is Needed.Cells · 2020Review
- Production of mouse offspring from inactivated spermatozoa using horse PLCζ mRNA.The Journal of reproduction and development · 2020Article
- Essential Role of Sperm-Specific PLC-Zeta in Egg Activation and Male Factor Infertility: An Update.Frontiers in cell and developmental biology · 2020Review
- The eggstraordinary story of how life begins.Molecular reproduction and development · 2019Review
- PLCζ is the physiological trigger of the CaDevelopment (Cambridge, England) · 2017Article
- Store-operated CaCell calcium · 2017Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Artificial oocyte activation to overcome failed fertilization after intracytoplasmic sperm injection (ICSI) in human oocytes typically employs Ca(2+) ionophores to produce a single cytosolic Ca(2+) increase. In contrast, recombinant phospholipase Czeta (PLCζ) causes Ca(2+) oscillations indistinguishable from those occurring during fertilization, but remains untested for its efficacy in a scenario of ICSI fertilization failure. Here, we compare PLCζ with other activation stimuli in a mouse model of failed oocyte activation after ICSI, in which heat-treated sperm are injected into mouse oocytes. We show that increasing periods of 56 °C exposure of sperm produces a progressive loss of Ca(2+) oscillations after ICSI. The decrease in Ca(2+) oscillations produces a reduction in oocyte activation and embryo development to the blastocyst stage. We treated such oocytes that failed to activate after ICSI either with Ca(2+) ionophore, or with Sr(2+) media which causes Ca(2+) oscillations, or we injected them with recombinant human PLCζ. All these treatments rescued oocyte activation, although Sr(2+) and PLCζ gave the highest rates of development to blastocyst. When recombinant PLCζ was given to oocytes previously injected with control sperm, they developed normally to the blastocyst stage at rates similar to that after control ICSI. The data suggest that recombinant human PLCζ protein is an efficient means of rescuing oocyte activation after ICSI failure and that it can be effectively used even if the sperm already contains endogenous Ca(2+) releasing activity.
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