ArticleWorld journal of microbiology & biotechnology2015
Rapid and qualitative fluorescence-based method for the assessment of PHA production in marine bacteria during batch culture.
Article in World journal of microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 31 citations in OpenAlex.
- Monitoring proliferation and material production of Cupriavidus necator H16 using cell count and volume measurement.Scientific reports · 2025Article
- Genome sequence of the marine alphaproteobacteriumMicrobiology resource announcements · 2024Article
- Microplastics a Novel Substratum for Polyhydroxyalkanoate (PHA)-Producing Bacteria in Aquatic Environments.Current microbiology · 2022Article
- High natural PHA production from acetate in Cobetia sp. MC34 and Cobetia marina DSM 4741Microbial cell factories · 2021Article
- Analysis of Polyhydroxyalkanoates Granules inPolymers · 2021Article
- The use of LipidGreen2 for visualization and quantification of intracellular Poly(3-hydroxybutyrate) inBiochemistry and biophysics reports · 2020Article
- Current developments on polyhydroxyalkanoates synthesis by using halophiles as a promising cell factory.Microbial cell factories · 2020Review
- PHA Production and PHA Synthases of the Halophilic BacteriumBioengineering (Basel, Switzerland) · 2020Article
- Complete genome sequence of the halophilic PHA-producing bacterium Halomonas sp. SF2003: insights into its biotechnological potential.World journal of microbiology & biotechnology · 2019Article
- Colonization of Non-biodegradable and Biodegradable Plastics by Marine Microorganisms.Frontiers in microbiology · 2018Article
- Investigations into the characterization, degradation, and applications of biodegradable polymers by mass spectrometry.Mass spectrometry reviewsReview
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The expansion of polyhydroxyalkanoates (PHAs) into the biodegradable polymers market is mainly prevented by their production process which is still complicated with a low efficiency, resulting in relatively expensive products. In this study, we developed a method that used the lipophilic fluorescent probe Nile Red (1 mg l(-1) solution in DMSO) directly into the culture broth to stain the PHA inclusions inside bacterial cells followed by detection of the emitted fluorescence by both microscopic and spectrometric techniques. Epifluorescence microscopy provides a rapid tool to distinguish producing from non-producing bacterial species and the relative fluorescence intensity (FI) determined at the maximum of emission spectra in the wavelength region of 560-710 nm (λ(ex): 543 nm), allows a fast assessment of the cultural conditions that may enhance PHA production yield. During two-step cultivation in 500-ml flasks with glucose as the sole carbon source, the method aimed to select bacterial strains efficient for PHA synthesis among a marine collection. Subsequently, the NR assay was used to determine the C0/N0 ratio of the producing media that may improve the polymer yield as well as to follow the time course of fermentation. Characterization by GC-MS and DSC confirmed the production of the P(3-HB) homopolymer.
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