Evidence map›Paper›PMID 26187057›Full record

ArticleBurns : journal of the International Society for Burn Injuries2015

Rapid creation of skin substitutes from human skin cells and biomimetic nanofibers for acute full-thickness wound repair.

Seyed Babak Mahjour, Xiaoling Fu, Xiaochuan Yang, Jason Fong, Farshid Sefat, Hongjun Wang

Open access · greenAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Burns : journal of the International Society for Burn Injuries, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.

0numbers the graph read from it
0cells of the map it votes in
16citing papers in PubMed
2.7field-weighted citation impact, top 12% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

16 citing papers in PubMed, 62 citations in OpenAlex.

  1. Review
  2. Review
  3. Review
  4. Article
  5. Stem Cell Niche Microenvironment: Review.Bioengineering (Basel, Switzerland) · 2021
    Review
  6. Characterization of nanofibers for tissue engineering: Chemical mapping by Confocal Raman microscopy.Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy · 2020
    Article
  7. Review
  8. Article
  9. Article
  10. Article
  11. Article
  12. Review
  13. Article
  14. Bioresorbable scaffold as a dermal substitute.International journal of burns and trauma · 2017
    Article
  15. Review
  16. Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 3 countries.

Seyed Babak MahjourDepartment of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
Xiaoling FuDepartment of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA; Department of Biomedical Engineering, School of Materials Science and Engineering, South China University of Technology, Guangzhou, China.
Xiaochuan YangDepartment of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
Jason FongDepartment of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA.
Farshid SefatDepartment of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA; Department of Biomedical Engineering, School of Engineering, King Faisal University, Al-Hofuf, Al-Ahsa, Saudi Arabia.
Hongjun WangDepartment of Chemistry, Chemical Biology and Biomedical Engineering, Stevens Institute of Technology, Hoboken, NJ 07030, USA. Electronic address: Hongjun.Wang@stevens.edu.
Stevens Institute of Technology · USSouth China University of Technology · CN

Funding

Rapid creation of autologous skin substitutes for wound repairR21AR056416 · NIAMS · THE TRUSTEES OF THE STEVENS INSTITUTE OF TECHNOLOGY · PI WANG, HONGJUN · 2009 to 2010
$382k
NIAMS NIH HHS 1R21 AR056416NIAMS NIH HHS R21 AR056416
6 · The paper itself

Abstract

Creation of functional skin substitutes within a clinically acceptable time window is essential for timely repair and management of large wounds such as extensive burns. The aim of this study was to investigate the possibility of fabricating skin substitutes via a bottom-up nanofiber-enabled cell assembly approach and using such substitutes for full-thickness wound repair in nude mice. Following a layer-by-layer (L-b-L) manner, human primary skin cells (fibroblasts and keratinocytes) were rapidly assembled together with electrospun polycaprolactone (PCL)/collagen (3:1, w/w; 8%, w/v) nanofibers into 3D constructs, in which fibroblasts and keratinocytes were located in the bottom and upper portion respectively. Following culture, the constructs developed into a skin-like structure with expression of basal keratinocyte markers and deposition of new matrix while exhibiting good mechanical strength (as high as 4.0 MPa by 14 days). Treatment of the full-thickness wounds created on the back of nude mice with various grafts (acellular nanofiber meshes, dermal substitutes, skin substitutes and autografts) revealed that 14-day-cultured skin substitutes facilitated a rapid wound closure with complete epithelialization comparable to autografts. Taken together, skin-like substitutes can be formed by L-b-L assembling human skin cells and biomimetic nanofibers and they are effective to heal acute full-thickness wounds in nude mice.

Indexed as

FibroblastsKeratinocytesSkin, ArtificialAcellular DermisAnimalsBiomimetic MaterialsCell ProliferationForeskinHumansInfant, NewbornMaleMiceMice, NudeNanofibersPolyestersSkinpolycaprolactonePolyestersLayer-by-layerPCL/collagen polyblend nanofibersSkin tissue engineeringWound healing

Identifiers

PMID26187057
PMCPMC5712430
OpenAlexW929827579

What OpenQuestion holds

Textmetadata
LicenceTDM
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.