ArticlePloS one2015
Correlation of Real Time PCR Cycle Threshold Cut-Off with Bordetella pertussis Clinical Severity.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers, 1 of them a synthesis that pooled it.
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Who cites it
16 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Systematic review on the association between respiratory virus real-time PCR cycle threshold values and clinical presentation or outcomes.The Journal of antimicrobial chemotherapy · 2021Pooled it
- BPZE1 vaccination induces IL-17NPJ vaccines · 2026Article
- Public health management of pertussis in adults: Practical challenges and future strategies.Human vaccines & immunotherapeutics · 2024Review
- Understanding the impact of adult pertussis and current approaches to vaccination: A narrative review and expert panel recommendations.Human vaccines & immunotherapeutics · 2024Review
- Association between real-time polymerase chain reaction cycle threshold value and clinical presentation in children with Bordetella pertussis.Italian journal of pediatrics · 2024Article
- Associations between methamphetamine use disorder andDialogues in clinical neuroscience · 2024Article
- Species-specific environmental DNA analysis of the index species in soil ecosystem,Ecology and evolution · 2022Article
- Estimating epidemiologic dynamics from cross-sectional viral load distributions.Science (New York, N.Y.) · 2021Article
- A Call for Caution in Use of Pertussis Vaccine Effectiveness Studies to Estimate Waning Immunity: A Canadian Immunization Research Network Study.Clinical infectious diseases : an official publication of the Infectious Diseases Society of America · 2021Article
- Estimating epidemiologic dynamics from cross-sectional viral load distributions.medRxiv : the preprint server for health sciences · 2021Article
- Tracheal colonization factor A (TcfA) is a biomarker for rapid and specific detection of Bordetella pertussis.Scientific reports · 2020Article
- Challenges and clinical relevance of molecular detection of Bordetella pertussis in South Africa.BMC infectious diseases · 2019Article
- Article
- Should equivocalCanada communicable disease report = Releve des maladies transmissibles au Canada · 2018Article
- Effectiveness of pertussis vaccination and duration of immunity.CMAJ : Canadian Medical Association journal = journal de l'Association medicale canadienne · 2016Article
- Application of species-specific primers to estimate theJournal of plankton researchArticle
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Bordetella pertussis testing performed using real-time polymerase chain reaction (RT-PCR) is interpreted based on a cycle threshold (Ct) value. At Public Health Ontario Laboratories (PHOL), a Ct value <36 is reported as positive, and Ct values ≥36 and <40 are reported as indeterminate. PHOL reported indeterminate results to physicians and public health units until May 2012, after which these results were only reported to physicians. We investigated the association between Ct value and disease symptom and severity to examine the significance of indeterminate results clinically, epidemiologically and for public health reporting. B. pertussis positive and indeterminate RT-PCR results were linked to pertussis cases reported in the provincial Integrated Public Health Information System (iPHIS), using deterministic linkage. Patients with positive RT-PCR results had a lower median age of 10.8 years compared to 12.0 years for patients with indeterminate results (p = 0.24). Hospitalized patients had significantly lower Ct values than non-hospitalized patients (median Ct values of 20.7 vs. 31.6, p<0.001). The proportion of patients reporting the most indicative symptoms of pertussis did not differ between patients with positive vs. indeterminate RT-PCR results. Taking the most indicative symptoms of pertussis as the gold-standard, the positive predictive value of the RT-PCR test was 68.1%. RT-PCR test results should be interpreted in the context of the clinical symptoms, age, vaccination status, prevalence, and other factors. Further information on interpretation of indeterminate RT-PCR results may be needed, and the utility of reporting to public health practitioners should be re-evaluated.
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