ArticleOrganogenesis2015
Creation and implantation of acellular rat renal ECM-based scaffolds.
Article in Organogenesis, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers, 1 of them a synthesis that pooled it.
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Who cites it
19 citing papers in PubMed, 1 synthesis or guideline pooled it, 50 citations in OpenAlex.
- Current strategies on kidney regeneration using tissue engineering approaches: a systematic review.BMC nephrology · 2025Pooled it
- Revolutionizing Renal Replacement: Current Advancements in Development and Transplantation of Bioengineered Kidneys.International journal of molecular sciences · 2026Review
- Towards Safety and Regulation Criteria for Clinical Applications of Decellularized Organ-Derived Matrices.Bioengineering (Basel, Switzerland) · 2025Review
- Heparin Immobilization Enhances Hemocompatibility, Re-Endothelization, and Angiogenesis of Decellularized Liver Scaffolds.International journal of molecular sciences · 2024Article
- Vascularization of kidney organoids: different strategies and perspectives.Frontiers in urology · 2024Review
- Capturing effects of blood flow on the transplanted decellularized nephron with intravital microscopy.Scientific reports · 2023Article
- Sustained in vivo perfusion of a re-endothelialized tissue engineered kidney graft in a human-scale animal model.Frontiers in bioengineering and biotechnology · 2023Article
- Mesenchymal Stromal Cell on Liver Decellularised Extracellular Matrix for Tissue Engineering.Biomedicines · 2022Article
- Decellularized blood vessel development: Current state-of-the-art and future directions.Frontiers in bioengineering and biotechnology · 2022Review
- Bioengineered Kidney Models: Methods and Functional Assessments.Function (Oxford, England) · 2021Review
- Kidney tissue engineering using a well-preserved acellular rat kidney scaffold and mesenchymal stem cells.Veterinary research forum : an international quarterly journal · 2021Article
- Wnt ligands 3a and 5a regulate proliferation and migration in human fetal liver progenitor cells.Translational gastroenterology and hepatology · 2021Article
- Accounting for Material Changes in Decellularized Tissue with Underutilized Methodologies.BioMed research international · 2021Review
- Human ucMSCs seeded in a decellularized kidney scaffold attenuate renal fibrosis by reducing epithelial-mesenchymal transition via the TGF-β/Smad signaling pathway.Pediatric research · 2020Article
- PKHCells · 2020Article
- Formation and optimization of three-dimensional organoids generated from urine-derived stem cells for renal function in vitro.Stem cell research & therapy · 2020Article
- A Comparative Study of the Effects of Different Decellularization Methods and Genipin-Cross-Linking on the Properties of Tracheal Matrices.Tissue engineering and regenerative medicine · 2019Article
- Experimental Evaluation of Kidney Regeneration by Organ Scaffold Recellularization.Scientific reports · 2017Article
- Re-epithelialization of whole porcine kidneys with renal epithelial cells.Journal of tissue engineeringArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
15 authors at 7 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Kidney transplantation is the only potentially curative treatment for patient facing end-stage renal disease, and it is now routinely used. Its use is mainly limited by the supply of transplantable donor organs, which far exceeds the demand. Regenerative medicine and tissue engineering offer promising means for overcoming this shortage. In the present study, we developed and validated a protocol for producing acellular rat renal scaffolds. Left kidneys were removed from 26 male Lewis rats (weights: 250-350 g) and decellularized by means of aortic anterograde perfusion with ionic and anionic detergents (Triton X-100 1% and SDS 1%, respectively). 19 scaffolds thus obtained (and contralateral native kidneys as controls) were deeply characterized in order to evaluate the decellularization quality, the preservation of extracellular matrix components and resultant micro-angioarchitecture structure. The other 7 were transplanted into 7 recipient rats that had undergone unilateral nephrectomy. Recipients were sacrificed on post-transplantation day 7 and the scaffolds subjected to histologic studies. The dual-detergent protocol showed, with only 5 h of perfusion per organ, to obtain thoroughly decellularized renal scaffolds consisting almost exclusively of extracellular matrix. Finally the macro- and the microarchitecture of the renal parenchyma were well preserved, and the grafts were implanted with ease. Seven days after transplant, the scaffolds were morphologically intact although all vascular structures were obstructed with thrombi. Production and implantation of acellular rat renal scaffolds is a suitable platform for further studies on regenerative medicine and tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.