ArticleToxicological sciences : an official journal of the Society of Toxicology2015
Ethanol Attenuates Histiotrophic Nutrition Pathways and Alters the Intracellular Redox Environment and Thiol Proteome during Rat Organogenesis.
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 15 citations in OpenAlex.
- The plasma metabolome of women in early pregnancy differs from that of non-pregnant women.PloS one · 2019Trial
- Prenatal alcohol exposure affects placental degradation-a retrospective fetal MRI study.European radiology · 2026Article
- Spatiotemporal evaluation of the mouse embryonic redox environment and histiotrophic nutrition following treatment with valproic acid and 1,2-dithiole-3-thione during early organogenesis.Reproductive toxicology (Elmsford, N.Y.) · 2021Article
- The Redox Theory of Development.Antioxidants & redox signaling · 2020Review
- A mechanistic model for thiol redox dynamics in the organogenesis stage rat conceptus.Reproductive toxicology (Elmsford, N.Y.) · 2018Article
- Early-Ethanol Exposure Induced Region-Specific Changes in Metabolic Proteins in the Rat Brain: A Proteomics Study.Journal of molecular neuroscience : MN · 2018Article
- Proceedings of the 2017 annual meeting of the Fetal Alcohol Spectrum Disorders study group.Alcohol (Fayetteville, N.Y.) · 2018Article
- Mono-2-ethylhexyl phthalate disrupts neurulation and modifies the embryonic redox environment and gene expression.Reproductive toxicology (Elmsford, N.Y.) · 2016Article
- Mono-2-ethylhexyl phthalate (MEHP) alters histiotrophic nutrition pathways and epigenetic processes in the developing conceptus.The Journal of nutritional biochemistry · 2016Article
- Amino acid starvation induced by protease inhibition produces differential alterations in redox status and the thiol proteome in organogenesis-stage rat embryos and visceral yolk sacs.The Journal of nutritional biochemistry · 2015Article
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Authors and funding
7 authors at 3 institutions in 1 country.
Funding
Abstract
Ethanol (EtOH) is a reactive oxygen-generating teratogen involved in the etiology of structural and functional developmental defects. Embryonic nutrition, redox environment, and changes in the thiol proteome following EtOH exposures (1.56.0 mg/ml) were studied in rat whole embryo culture. Glutathione (GSH) and cysteine (Cys) concentrations with their respective intracellular redox potentials (Eh) were determined using high-performance liquid chromatography. EtOH reduced GSH and Cys concentrations in embryo (EMB) and visceral yolk sac (VYS) tissues, and also in yolk sac and amniotic fluids. These changes produced greater oxidation as indicated by increasingly positive Eh values. EtOH reduced histiotrophic nutrition pathway activities as measured by the clearance of fluorescin isothiocyanate (FITC)-albumin from culture media. A significant decrease in total FITC clearance was observed at all concentrations, reaching approximately 50% at the highest dose. EtOH-induced changes to the thiol proteome were measured in EMBs and VYSs using isotope-coded affinity tags. Decreased concentrations for specific proteins from cytoskeletal dynamics and endocytosis pathways (α-actinin, α-tubulin, cubilin, and actin-related protein 2); nuclear translocation (Ran and RanBP1); and maintenance of receptor-mediated endocytosis (cubilin) were observed. Kyoto encyclopedia of genes and genomes (KEGG) pathway analysis also identified a decrease in ribosomal proteins in both EMB and VYS. Results show that EtOH interferes with nutrient uptake to reduce availability of amino acids and micronutrients required by the conceptus. Intracellular antioxidants such as GSH and Cys are depleted following EtOH and Eh values increase. Thiol proteome analysis in the EMB and VYS show selectively altered actin/cytoskeleton, endocytosis, ribosome biogenesis and function, nuclear transport, and stress-related responses.
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