ArticlePathogens and global health2015
Assessment of Legionella pneumophila in recreational spring water with quantitative PCR (Taqman) assay.
Article in Pathogens and global health, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed, 5 citations in OpenAlex.
- Review
- Cooccurrence of Five PathogenicPathogens (Basel, Switzerland) · 2021Article
- Droplet digital PCR for the detection and monitoring of Legionella pneumophila.International journal of molecular medicine · 2020Article
- Relationships between Microbial Indicators and Pathogens in Recreational Water Settings.International journal of environmental research and public health · 2018Review
- Potential human pathogenic bacteria in five hot springs in Eritrea revealed by next generation sequencing.PloS one · 2018Article
- Approach to determine the diversity of Legionella species by nested PCR-DGGE in aquatic environments.PloS one · 2017Article
Corrections and comments
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Legionella spp. are common in various natural and man-made aquatic environments. Recreational hot spring is frequently reported as an infection hotspot because of various factors such as temperature and humidity. Although polymerase chain reaction (PCR) had been used for detecting Legionella, several inhibitors such as humic substances, calcium, and melanin in the recreational spring water may interfere with the reaction thus resulting in risk underestimation. The purpose of this study was to compare the efficiencies of conventional and Taqman quantitative PCR (qPCR) on detecting Legionella pneumophila in spring facilities and in receiving water. In the results, Taqman PCR had much better efficiency on specifying the pathogen in both river and spring samples. L. pneumophila was detected in all of the 27 river water samples and 45 of the 48 hot spring water samples. The estimated L. pneumophela concentrations ranged between 1.0 × 10(2) and 3.3 × 10(5) cells/l in river water and 72.1-5.7 × 10(6) cells/l in hot spring water. Total coliforms and turbidity were significantly correlated with concentrations of L. pneumophila in positive water samples. Significant difference was also found in water temperature between the presence/absence of L. pneumophila. Our results suggest that conventional PCR may be not enough for detecting L. pneumophila particularly in the aquatic environments full of reaction inhibitors.
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