ArticleNeurochemical research2015
Dynamic Changes in Cytosolic ATP Levels in Cultured Glutamatergic Neurons During NMDA-Induced Synaptic Activity Supported by Glucose or Lactate.
Article in Neurochemical research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 27 citations in OpenAlex.
- Direct energy consumption by inhibitory GABAFrontiers in neuroscience · 2026Article
- Presynaptic ATP Decreases During Physiological-Like Activity in Neurons Tuned for High-Frequency Transmission.Journal of neurochemistry · 2025Article
- Unveiling the role of local metabolic constraints on the structure and activity of spiking neural networks.PLoS computational biology · 2025Article
- Spreading depolarizations exhaust neuronal ATP in a model of cerebral ischemia.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- The dentate gyrus differentially metabolizes glucose and alternative fuels during rest and stimulation.Journal of neurochemistry · 2024Article
- Variable bioenergetic sensitivity of neurons and astrocytes to insulin and extracellular glucose.npj metabolic health and disease · 2024Article
- Energy matters: presynaptic metabolism and the maintenance of synaptic transmission.Nature reviews. Neuroscience · 2022Review
- Developmental shift to mitochondrial respiration for energetic support of sustained transmission during maturation at the calyx of Held.Journal of neurophysiology · 2021Article
- Aerobic Glycolysis in the Brain: Warburg and Crabtree Contra Pasteur.Neurochemical research · 2021Review
- The Energy Homeostasis Principle: A Naturalistic Approach to Explain the Emergence of Behavior.Frontiers in systems neuroscience · 2021Article
- Intracellular ATP levels in mouse cortical excitatory neurons varies with sleep-wake states.Communications biology · 2020Article
- A Dual Nanosensor Approach to Determine the Cytosolic Concentration of ATP in Astrocytes.Frontiers in cellular neuroscience · 2020Article
- The Energy Homeostasis Principle: Neuronal Energy Regulation Drives Local Network Dynamics Generating Behavior.Frontiers in computational neuroscience · 2019Article
- Article
- Astrocytes, Metabolism, Signaling and Brain Drains: Introduction to the Special Issue in Honor of Gerald Dienel.Neurochemical research · 2015Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have previously shown that synaptic transmission fails in cultured neurons in the presence of lactate as the sole substrate. Thus, to test the hypothesis that the failure of synaptic transmission is a consequence of insufficient energy supply, ATP levels were monitored employing the ATP biosensor Ateam1.03YEMK. While inducing synaptic activity by subjecting cultured neurons to two 30 s pulses of NMDA (30 µM) with a 4 min interval, changes in relative ATP levels were measured in the presence of lactate (1 mM), glucose (2.5 mM) or the combination of the two. ATP levels reversibly declined following NMDA-induced neurotransmission activity, as indicated by a reversible 10-20 % decrease in the response of the biosensor. The responses were absent when the NMDA receptor antagonist memantine was present. In the presence of lactate alone, the ATP response dropped significantly more than in the presence of glucose following the 2nd pulse of NMDA (approx. 10 vs. 20 %). Further, cytosolic Ca(2+) homeostasis during NMDA-induced synaptic transmission is partially inhibited by verapamil indicating that voltage-gated Ca(2+) channels are activated. Lastly, we showed that cytosolic Ca(2+) homeostasis is supported equally well by both glucose and lactate, and that a pulse of NMDA causes accumulation of Ca(2+) in the mitochondrial matrix. In summary, we have shown that ATP homeostasis during neurotransmission activity in cultured neurons is supported by both glucose and lactate. However, ATP homeostasis seems to be negatively affected by the presence of lactate alone, suggesting that glucose is needed to support neuronal energy metabolism during activation.
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