Interaction of a novel fluorescent GTP analogue with the small G-protein K-Ras.

Seigo Iwata, Kaori Masuhara, Nobuhisa Umeki, Yasushi Sako, Shinsaku Maruta

Open access · bronzeAbstract read

In one paragraph

Article in Journal of biochemistry, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.2field-weighted citation impact, top 42% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 3 citations in OpenAlex.

Expression of Immuno-Oncologic Biomarkers Is Enriched in Colorectal Cancers and Other Solid Tumors Harboring the A59T Variant ofCells · 2021
Article

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors at 1 institution in 1 country.

Seigo IwataDivision of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan;

Kaori MasuharaDivision of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan;

Nobuhisa UmekiDivision of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan;

Yasushi SakoDivision of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan;

Shinsaku MarutaDivision of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan; Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577, Japan; maruta@soka.ac.jp.

Soka University · JP

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

A novel fluorescent guanosine 5'-triphosphate (GTP) analogue, 2'(3')-O-{6-(N-(7-nitrobenz-2-oxa-l,3-diazol-4-yl)amino) hexanoic}-GTP (NBD-GTP), was synthesized and utilized to monitor the effect of mutations in the functional region of mouse K-Ras. The effects of the G12S, A59T and G12S/A59T mutations on GTPase activity, nucleotide exchange rates were compared with normal Ras. Mutation at A59T resulted in reduction of the GTPase activity by 0.6-fold and enhancement of the nucleotide exchange rate by 2-fold compared with normal Ras. On the other hand, mutation at G12S only slightly affected the nucleotide exchange rate and did not affect the GTPase activity. We also used NBD-GTP to study the effect of these mutations on the interaction between Ras and SOS1, a guanine nucleotide exchange factor. The mutation at A59T abolished the interaction with SOS1. The results suggest that the fluorescent GTP analogue, NBD-GTP, is applicable to the kinetic studies for small G-proteins.

Indexed as

4-Chloro-7-nitrobenzofurazanAnimalsFluorescent DyesGuanine Nucleotide Exchange FactorsGuanosine TriphosphateKineticsMiceMonomeric GTP-Binding ProteinsMutationProtein BindingProto-Oncogene Proteins p21(ras)SOS1 Protein4-Chloro-7-nitrobenzofurazanFluorescent DyesGuanine Nucleotide Exchange FactorsGuanosine TriphosphateHras protein, mouseMonomeric GTP-Binding ProteinsProto-Oncogene Proteins p21(ras)SOS1 ProteinfluorescenceGEFG-proteinsnucleotide analogueRas

Identifiers

PMID26184075

DOI10.1093/jb/mvv071

PMCPMC4882638

OpenAlexW2312601058

What OpenQuestion holds

Textmetadata

Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.