ArticleScientific reports2015
Improved Oocyte Isolation and Embryonic Development of Outbred Deer Mice.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
9 citing papers in PubMed, 18 citations in OpenAlex.
- Testicular SIRT1 Loss Reveals an Aging-Like Proteomic Landscape and Precipitates Reproductive Deterioration.Andrology · 2026Article
- Hormone guided estrus synchronization using progesterone sponge and PMSG in goats: a cost-effective optimization strategy.Frontiers in veterinary science · 2026Article
- Optimized culture systems for the preimplantation ICR mouse embryos with wide range of EDTA concentrations.Royal Society open science · 2021Article
- Preconception Alcohol Exposure Increases the Susceptibility to Diabetes in the Offspring.Endocrinology · 2021Article
- Generation of embryonic stem cells derived from the inner cell mass of blastocysts of outbred ICR mice.Animal cells and systems · 2020Article
- Combined treatment with cysteamine and leukemia inhibitory factor promotes guinea pig oocyte meiosisAmerican journal of translational research · 2019Article
- Microfluidic Encapsulation of Ovarian Follicles for 3D Culture.Annals of biomedical engineering · 2017Review
- In vitro culture of ovarian follicles from Peromyscus.Seminars in cell & developmental biology · 2017Review
- The crucial role of zona pellucida in cryopreservation of oocytes by vitrification.Cryobiology · 2015Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In this study, we improved the protocol for isolating cumulus-oocyte complexes (COCs) from the outbred deer mice by using only one hormone (instead of the widely used combination of two hormones) with reduced dose. Moreover, we identified that significantly more metaphase II (MII) oocytes could be obtained by supplementing epidermal growth factor (EGF) and leukemia inhibition factor (LIF) into the previously established medium for in vitro maturation (IVM) of the COCs. Furthermore, we overcame the major challenge of two-cell block during embryonic development of deer mice after either in vitro fertilization (IVF) or parthenogenetic activation (PA) of the MII oocytes, by culturing the two-cell stage embryos on the feeder layer of inactivated mouse embryonic fibroblasts (MEFs) in the medium of mouse embryonic stem cells. Collectively, this work represents a major step forward in using deer mice as an outbred animal model for biomedical research on reproduction and early embryonic development.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.