Evidence map›Paper›PMID 26183951›Full record

ArticlePlant cell reports2015

Promoter analysis of the sweet potato ADP-glucose pyrophosphorylase gene IbAGP1 in Nicotiana tabacum.

Xuelian Zheng, Qian Li, Dongqing Liu, Lili Zang, Kaiyue Zhang, Kejun Deng, Shixin Yang, Zhengyang Xie, Xu Tang, Yiping Qi and 1 more

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Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact, top 84% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 5 citations in OpenAlex.

  1. Article
  2. Overexpression ofFrontiers in plant science · 2019
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4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors at 2 institutions in 2 countries.

Xuelian ZhengSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Qian LiSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Dongqing LiuSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Lili ZangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Kaiyue ZhangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Kejun DengSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Shixin YangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Zhengyang XieSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Xu TangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China.
Yiping QiDepartment of Biology, East Carolina University, Greenville, NC, 27858, USA. qiy@ecu.edu.
Yong ZhangSchool of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu, 610054, China. zhangyong916@uestc.edu.cn.
University of Electronic Science and Technology of China · CNEast Carolina University · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

key messageThe IbAGP1 gene of sweet potato ( Ipomoea batatas ) encodes the sucrose-inducible small subunit of ADP-glucose pyrophosphorylase. Through expression analysis of 5'-truncations and synthetic forms of the IbAGP1 promoter in transgenic tobacco, we show that SURE-Like elements and W-box elements of the promoter contribute to the sucrose inducibility of this gene. Sweet potato (Ipomoea batatas) contains two genes (IbAGP1 and IbAGP2) encoding the catalytically active small subunits of ADP-glucose pyrophosphorylase, an enzyme with an important role in regulating starch synthesis in higher plants. Previous studies have shown that IbAGP1 is expressed in the storage roots, leaves, and stem tissues of sweet potato, and its transcript is strongly induced by applying sucrose exogenously to detached leaves. To investigate the tissue-specific expression of the IbAGP1 promoter, a series of 5'-truncated promoters extending from bases -1913, -1598, -1298, -1053, -716, and -286 to base +75 were used to drive the expression of the β-glucuronidase reporter gene (GUS) in tobacco plants (Nicotiana tabacum). Histochemical and fluorometric GUS assays showed that (1) GUS expression driven by the longest fragment (1989 bp) of the IbAGP1 promoter was detected in vegetative tissues (roots, stems, leaves), (2) fragments extending to -1053 or beyond retained strong GUS expression in roots, stems, and leaves, whereas further 5'-deletions resulted in considerable reduction in GUS activity, and (3) the series of 5'-truncated promoters responded differently to exogenously applied sucrose. The 1989-bp IbAGP1 promoter contains five sequences (two AATAAAA, one AATAAAAAA, and two AATAAATAAA) that are similar to sucrose-responsive elements (SURE). These SURE-Like sequences are found at nucleotide positions -1273, -1239, -681, -610, and -189. Moreover, putative W-box elements are found at positions -1985, -1434, -750, and -578. Synthetic promoters containing tandem repeats of the 4X SURE-Like or 4X W-box upstream from a minimal CaMV35S promoter-GUS fusion showed significant expression in transgenic tobacco in response to exogenous sucrose. These results show that SURE-Like elements and W-box elements of the IbAGP1 promoter contribute to the sucrose inducibility of this gene.

Indexed as

Gene Expression Regulation, PlantGlucose-1-Phosphate AdenylyltransferaseIpomoea batatasNicotianaPlant ProteinsPlants, Genetically ModifiedPromoter Regions, GeneticGlucose-1-Phosphate AdenylyltransferasePlant ProteinsGUS expressionIbAGP1Ipomoea batatasNicotiana tabacumPromoter analysis

Identifiers

PMID26183951
OpenAlexW2472341724

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.