ArticleMolecular & cellular proteomics : MCP2015
A Pneumococcal Protein Array as a Platform to Discover Serodiagnostic Antigens Against Infection.
Article in Molecular & cellular proteomics : MCP, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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11 citing papers in PubMed, 17 citations in OpenAlex.
- Identification ofJournal of proteome research · 2025Article
- BacScan: a novel genome-wide strategy for uncovering broadly immunogenic proteins in bacteria.Frontiers in immunology · 2024Article
- Extracellular Vesicles from Different Pneumococcal Serotypes Are Internalized by Macrophages and Induce Host Immune Responses.Pathogens (Basel, Switzerland) · 2021Article
- Approaching In Vivo Models of Pneumococcus-Host Interaction: Insights into Surface Proteins, Capsule Production, and Extracellular Vesicles.Pathogens (Basel, Switzerland) · 2021Article
- Glass Slide-Printed Protein Arrays as a Platform to Discover Serodiagnostic Antigens Against Bacterial Infections.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Diagnosis of Streptococcus pneumoniae infection using circulating antibody secreting cells.PloS one · 2021Article
- Proteomic and Bioinformatic Analysis ofVaccines · 2020Article
- Prediction and Validation of Immunogenic Domains of Pneumococcal Proteins Recognized by Human CD4Infection and immunity · 2019Article
- Article
- Integrated proteomic and metabolomic analysis reveals that rhodomyrtone reduces the capsule in Streptococcus pneumoniae.Scientific reports · 2017Article
- Data in support of proteomic analysis of pneumococcal pediatric clinical isolates to construct a protein array.Data in brief · 2016Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pneumonia is one of the most common and severe diseases associated with Streptococcus pneumoniae infections in children and adults. Etiological diagnosis of pneumococcal pneumonia in children is generally challenging because of limitations of diagnostic tests and interference with nasopharyngeal colonizing strains. Serological assays have recently gained interest to overcome some problems found with current diagnostic tests in pediatric pneumococcal pneumonia. To provide insight into this field, we have developed a protein array to screen the antibody response to many antigens simultaneously. Proteins were selected by experimental identification from a collection of 24 highly prevalent pediatric clinical isolates in Spain, using a proteomics approach consisting of "shaving" the cell surface with proteases and further LC/MS/MS analysis. Ninety-five proteins were recombinantly produced and printed on an array. We probed it with a collection of sera from children with pneumococcal pneumonia. From the set of the most seroprevalent antigens, we obtained a clear discriminant response for a group of three proteins (PblB, PulA, and PrtA) in children under 4 years old. We validated the results by ELISA and an immunostrip assay showed the translation to easy-to-use, affordable tests. Thus, the protein array here developed presents a tool for broad use in serodiagnostics.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.