Evidence map›Paper›PMID 26182859›Full record

ArticleScientific reports2015

Characterization of species-specific genes regulated by E2-2 in human plasmacytoid dendritic cells.

Menglan Cheng, Xuyuan Zhang, Haisheng Yu, Peishuang Du, Joël Plumas, Laurance Chaperot, Lishan Su, Liguo Zhang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed
0.6field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 19 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Cell-Type Deconvolution Reveals Dynamic Changes in MASLD.Liver international communications · 2025
    Article
  5. Review
  6. Development and function of chicken XCR1Frontiers in immunology · 2023
    Article
  7. Article
  8. Review
  9. Article
  10. Type I Interferon Production of Plasmacytoid Dendritic Cells under Control.International journal of molecular sciences · 2021
    Review
  11. Article
  12. Review
  13. Article
  14. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 2 institutions in 2 countries.

Menglan ChengKey Laboratory of Immunity and Infection, Institute of Biophysics, University of Chinese Academy of Sciences, Beijing, China.
Xuyuan ZhangKey Laboratory of Immunity and Infection, Institute of Biophysics, University of Chinese Academy of Sciences, Beijing, China.
Haisheng YuKey Laboratory of Immunity and Infection, Institute of Biophysics, University of Chinese Academy of Sciences, Beijing, China.
Peishuang DuKey Laboratory of Immunity and Infection, Institute of Biophysics, University of Chinese Academy of Sciences, Beijing, China.
Joël PlumasDepartment of Research and Development, EFS Rh ône-Alpes Grenoble, La Tronche, France.
Laurance ChaperotDepartment of Research and Development, EFS Rh ône-Alpes Grenoble, La Tronche, France.
Lishan Su1] Key Laboratory of Immunity and Infection, Institute of Biophysics, University of Chinese Academy of Sciences, Beijing, China [2] Lineberger Comprehensive Cancer Center, Department of Microbiology and Immunology, School of Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Liguo ZhangKey Laboratory of Immunity and Infection, Institute of Biophysics, University of Chinese Academy of Sciences, Beijing, China.
Institute of Biophysics · CNUniversity of North Carolina at Chapel Hill · US

Funding

Murine Model of HCV-Associated Human Liver CancerR01CA164029 · NCI · UNIV OF NORTH CAROLINA CHAPEL HILL · PI LEMON, STANLEY M., SU, LISHAN · 2012 to 2016
$2.3M
HIV co-infection and HCV-induced liver fibrosis in vivoR01AI095097 · NIAID · UNIV OF NORTH CAROLINA CHAPEL HILL · PI SU, LISHAN · 2012 to 2016
$1.8M
NCI NIH HHS R01 CA164029NIAID NIH HHS R01 AI095097
6 · The paper itself

Abstract

Dendritic cells (DCs) are sentinels of the immune system and comprise two distinct subsets: conventional DCs (cDCs) and plasmacytoid DCs (pDCs). Human pDCs are distinguished from mouse pDCs phenotypically and functionally. Basic helix-loop-helix protein E2-2 is defined as an essential transcription factor for mouse pDC development, cell fate maintenance and gene programe. It is unknown whether E2-2 regulation contributes to this species-specific difference. Here we investigated the function of E2-2 in human pDCs and screened human-specific genes regulated by E2-2. Reduced E2-2 expression in human pDC cell line GEN2.2 resulted in diminished IFN-α production in response to CpG but elevated antigen presentation capacity. Gene expression profiling showed that E2-2 silence down-regulated pDC signature genes but up-regulated cDC signature genes. Thirty human-specific genes regulated by E2-2 knockdown were identified. Among these genes, we confirmed that expression of Siglec-6 was inhibited by E2-2. Further more, Siglec-6 was expressed at a higher level on a human pDC subset with drastically lower expression of E2-2. Collectively, these results highlight that E2-2 modulates pDC function in a species-specific manner, which may provide insights for pDC development and functions.

Indexed as

Gene Expression RegulationAnimalsCell LineCytokinesDendritic CellsGene Expression ProfilingGene Knockdown TechniquesGene SilencingHumansInterferon-alphaLymphocyte ActivationMiceRNA, Small InterferingSpecies SpecificityT-Lymphocyte SubsetsTranscription Factor 7-Like 2 ProteinCytokinesInterferon-alphaRNA, Small InterferingTCF7L2 protein, humanTranscription Factor 7-Like 2 Protein

Identifiers

PMID26182859
PMCPMC4505321
OpenAlexW2261772337

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.