Evidence map›Paper›PMID 26182374›Full record

ArticleBioscience reports2015

Does the liposuction method influence the phenotypic characteristic of human adipose-derived stem cells?

Anna Bajek, Natalia Gurtowska, Lidia Gackowska, Izabela Kubiszewska, Magdalena Bodnar, Andrzej Marszałek, Rafał Januszewski, Jacek Michalkiewicz, Tomasz Drewa

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In one paragraph

Article in Bioscience reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed.

  1. Article
  2. Review
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  4. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors.

Anna BajekDepartment of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland a_bajek@wp.pl.
Natalia GurtowskaDepartment of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland.
Lidia GackowskaChair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
Izabela KubiszewskaChair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
Magdalena BodnarDepartment of Clinical Pathomorphology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
Andrzej MarszałekDepartment of Clinical Pathomorphology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland.
Rafał JanuszewskiDepartment of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland.
Jacek MichalkiewiczChair of Immunology, Nicolaus Copernicus University, 85-094 Bydgoszcz, Poland Department of Microbiology and Immunology, The Children's Memorial Health Institute, 04-730 Warsaw, Poland.
Tomasz DrewaDepartment of Tissue Engineering, Nicolaus Copernicus University, 85-092 Bydgoszcz, Poland Department of Urology, Nicolaus Copernicus Hospital, 87-100 Torun, Poland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Adipose-derived stem cells (ASCs) possess a high differentiation and proliferation potential. However, the phenotypic characterization of ASCs is still difficult. Until now, there is no extensive analysis of ASCs markers depending on different liposuction methods. Therefore, the aim of the present study was to analyse 242 surface markers and determine the differences in the phenotypic pattern between ASCs obtained during mechanical and ultrasound-assisted liposuction. ASCs were isolated from healthy donors, due to mechanical and ultrasound-assisted liposuction and cultured in standard medium to the second passage. Differentiation potential and markers expression was evaluated to confirm the mesenchymal nature of cells. Then, the BD LyoplateTM Human Cell Surface Marker Screening Panel was used. Results shown that both population of ASCs are characterized by high expression of markers specific for ASCs: cluster of differentiation (CD)9, CD10, CD34, CD44, CD49d, CD54, CD55, CD59, CD71 and low expression of CD11a, CD11c and CD144. Moreover, we have noticed significant differences in antigen expression in 58 markers from the 242 studied. Presented study shows for the first time that different liposuction methods are not a significant factor which can influence the expression of human ASCs surface markers.

Indexed as

AdipocytesAdipogenesisAdipose TissueAdultAntigens, CDBiomarkersCell DifferentiationCells, CulturedHumansLipectomyOsteogenesisPhenotypeStem CellsUltrasonography, InterventionalAntigens, CDBiomarkersadipose-derived stem cellsliposuctionmarkers' extensive analysisphenotypic characterization

Identifiers

PMID26182374
PMCPMC4613685

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.