Evidence map›Paper›PMID 26181106›Full record

ArticleEndocrinology2015

Transcriptional and Functional Characterization of the G Protein-Coupled Receptor Repertoire of Gastric Somatostatin Cells.

Kristoffer L Egerod, Maja S Engelstoft, Mari L Lund, Kaare V Grunddal, Mirabella Zhao, Dominique Barir-Jensen, Eva B Nygaard, Natalia Petersen, Jens J Holst, Thue W Schwartz

Open access · bronzeAbstract read
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In one paragraph

Article in Endocrinology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.

0numbers the graph read from it
0cells of the map it votes in
31citing papers in PubMed
3.5field-weighted citation impact, top 7% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

31 citing papers in PubMed, 64 citations in OpenAlex.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

10 authors at 2 institutions in 1 country.

Kristoffer L EgerodNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Maja S EngelstoftNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Mari L LundNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Kaare V GrunddalNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Mirabella ZhaoNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Dominique Barir-JensenNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Eva B NygaardNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Natalia PetersenNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Jens J HolstNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
Thue W SchwartzNovo Nordisk Foundation Center for Basic Metabolic Research (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., J.J.H., T.W.S.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Laboratory for Molecular Pharmacology (K.L.E., M.S.E., M.L.L., K.V.G., M.Z., D.B.-J., E.B.N., N.P., T.W.S.), Department of Neuroscience and Pharmacology, Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark; Danish Diabetes Academy (M.S.E.), 5000 Odense, Denmark; and Department of Biomedical Sciences (J.J.H.), Faculty of Health and Medical Sciences, University of Copenhagen, 2200 Copenhagen, Denmark.
University of Copenhagen · DKNovo Nordisk Foundation · DK

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

In the stomach, somatostatin (SST) acts as a general paracrine negative regulator of exocrine secretion of gastric acid and pepsinogen and endocrine secretion of gastrin, ghrelin, and histamine. Using reporter mice expressing red fluorescent protein (RFP) under control of the SST promotor, we have characterized the G protein-coupled receptors expressed in gastric Sst-RFP-positive cells and probed their effects on SST secretion in primary cell cultures. Surprisingly, besides SST, amylin and PYY were also highly enriched in the SST cells. Several receptors found to regulate SST secretion were highly expressed and/or enriched. 1) The metabolite receptors calcium-sensing receptor and free fatty acid receptor 4 (GPR120) functioned as positive and negative regulators, respectively. 2) Among the neurotransmitter receptors, adrenergic receptors α1a, α2a, α2b, and β1 were all highly expressed, with norepinephrine and isoproterenol acting as positive regulators. The muscarinic receptor M3 acted as a positive regulator, whereas M4 was conceivably a negative regulator. 3) Of the hormone receptors, the GLP-1 and GIP receptors, CCKb (stimulated by both CCK and gastrin) and surprisingly the melanocortin MC1 receptor were all positive regulators. 4) The neuropeptide receptors for calcitonin gene-related peptide, adrenomedullin, and vasoactive intestinal peptide acted as positive regulators, no effect was observed using galanin and nociceptin although transcripts for the corresponding receptors appeared highly expressed. 5) The SST receptors 1 and 2 functioned in an autocrine negative feedback loop. Thus, the article provides a comprehensive map of receptors through which SST secretion is regulated by hormones, neurotransmitters, neuropeptides and metabolites that act directly on the SST cells in the gastric mucosa.

Indexed as

TranscriptomeAnimalsCells, CulturedGastric MucosaGlucagon-Like Peptide-1 ReceptorHumansIn Situ HybridizationIslet Amyloid PolypeptideLuminescent ProteinsMaleMice, KnockoutMice, TransgenicMicroscopy, ConfocalPeptide YYReceptors, AdrenergicReceptors, G-Protein-CoupledFFAR4 protein, mouseGlucagon-Like Peptide-1 ReceptorIslet Amyloid PolypeptideLuminescent ProteinsPeptide YYReceptors, AdrenergicReceptors, G-Protein-CoupledReceptors, MuscarinicReceptors, NeuropeptideReceptors, SomatostatinRed Fluorescent ProteinSomatostatin

Identifiers

PMID26181106
OpenAlexW1797882464

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.