ArticleMolecular biology of the cell2015
Contributions of epsinR and gadkin to clathrin-mediated intracellular trafficking.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 41 citations in OpenAlex.
- Article
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- Passive diffusion accounts for the majority of intracellular nanovesicle transport.Life science alliance · 2024Article
- Protein sorting from endosomes to the TGN.Frontiers in cell and developmental biology · 2023Review
- Endomembranes promote chromosome missegregation by ensheathing misaligned chromosomes.The Journal of cell biology · 2022Article
- Retrograde transport of CDMPR depends on several machineries as analyzed by sulfatable nanobodies.Life science alliance · 2022Article
- Cytoplasmic dynein-1 cargo diversity is mediated by the combinatorial assembly of FTS-Hook-FHIP complexes.eLife · 2021Article
- An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Spatial proteomics defines the content of trafficking vesicles captured by golgin tethers.Nature communications · 2020Article
- Identification of Podocyte Cargo Proteins by Proteomic Analysis of Clathrin-Coated Vesicles.Kidney360 · 2020Article
- Tumor protein D54 defines a new class of intracellular transport vesicles.The Journal of cell biology · 2020Article
- Fast and cloning-free CRISPR/Cas9-mediated genomic editing in mammalian cells.Traffic (Copenhagen, Denmark) · 2019Article
- Cargo Sorting at theCells · 2019Review
- Visualization of Protein Sorting at theFrontiers in cell and developmental biology · 2019Article
- A mechanism for differential sorting of the planar cell polarity proteins Frizzled6 and Vangl2 at theThe Journal of biological chemistry · 2018Article
- Light-activated protein interaction with high spatial subcellular confinement.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The WDR11 complex facilitates the tethering of AP-1-derived vesicles.Nature communications · 2018Article
- TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the trans-Golgi.Nature cell biology · 2017Article
- Contribution of the clathrin adaptor AP-1 subunit µ1 to acidic cluster protein sorting.The Journal of cell biology · 2017Article
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
Abstract
The precise functions of most of the proteins that participate in clathrin-mediated intracellular trafficking are unknown. We investigated two such proteins, epsinR and gadkin, using the knocksideways method, which rapidly depletes proteins from the available pool by trapping them onto mitochondria. Although epsinR is known to be an N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE)-specific adaptor, the epsinR knocksideways blocked the production of the entire population of intracellular clathrin-coated vesicles (CCVs), suggesting a more global function. Using the epsinR knocksideways data, we were able to estimate the copy number of all major intracellular CCV proteins. Both sides of the vesicle are densely covered, indicating that CCVs sort their cargo by molecular crowding. Trapping of gadkin onto mitochondria also blocked the production of intracellular CCVs but by a different mechanism: vesicles became cross-linked to mitochondria and pulled out toward the cell periphery. Both phenotypes provide new insights into the regulation of intracellular CCV formation, which could not have been found using more conventional approaches.
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