ArticlePlant cell reports2015
Selection of reference genes for quantitative real-time PCR normalization in creeping bentgrass involved in four abiotic stresses.
Article in Plant cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 73 citations in OpenAlex.
- Identification and Validation of Reference Genes for Reliable RT-qPCR Normalization inPlants (Basel, Switzerland) · 2026Article
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- Genome-wide identification, characterization, and functional analysis of the CHX, SOS, and RLK genes in Solanum lycopersicum under salt stress.Scientific reports · 2025Article
- The Expression Profile of Genes Related to Carotenoid Biosynthesis in Pepper Under Abiotic Stress Reveals a Positive Correlation with Plant Tolerance.Life (Basel, Switzerland) · 2024Article
- Differential Gene Expression Responses to Salt and Drought Stress in Tall Fescue (Festuca arundinacea Schreb.).Molecular biotechnology · 2024Article
- Comparative proteomics in tall fescue to reveal underlying mechanisms for improving Photosystem II thermotolerance during heat stress memory.BMC genomics · 2024Article
- Systematic selection of suitable reference genes for quantitative real-time PCR normalization studies of gene expression in Lutjanus erythropterus.Scientific reports · 2024Article
- Genome-wide identification and evolutionary analysis of the AP2/EREBP, COX and LTP genes in Zea mays L. under drought stress.Scientific reports · 2024Article
- Characterizing reference genes for high-fidelity gene expression analysis under different abiotic stresses and elicitor treatments in fenugreek leaves.Plant methods · 2024Article
- Identification of Accurate Reference Genes for qRT-PCR Analysis of Gene Expression inPlants (Basel, Switzerland) · 2023Article
- Selection and validation of appropriate reference genes for RT-qPCR analysis of Nitraria sibirica under various abiotic stresses.BMC plant biology · 2022Article
- The Barley Heavy Metal Associated Isoprenylated Plant Protein HvFP1 Is Involved in a Crosstalk between the Leaf Development and Abscisic Acid-Related Drought Stress Responses.Plants (Basel, Switzerland) · 2022Article
- Article
- Reference gene selection for quantitative RT-PCR in Miscanthus sacchariflorus under abiotic stress conditions.Molecular biology reports · 2022Article
- Identification of reliable reference genes for quantitative real-time PCR analysis of the Rhus chinensis Mill. leaf response to temperature changes.FEBS open bio · 2021Article
- Article
- Selection and Validation of Reference Genes for Quantitative Real-Time PCR in White Clover (Plants (Basel, Switzerland) · 2020Article
- Selection and Validation of Reference Genes for Normalisation of Gene Expression in Glehnia littoralis.Scientific reports · 2020Article
- Identification and validation of superior housekeeping gene(s) for qRT-PCR data normalization inPhysiology and molecular biology of plants : an international journal of functional plant biology · 2020Article
- Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
key messageThis study identified stable reference genes for normalization of gene expression data in qRT-PCR analysis of leaf and root tissues in creeping bentgrass under four abiotic stresses. Examination of gene expression using quantitative real-time PCR (qRT-PCR) in plant responses to abiotic stresses can provide valuable information for stress-tolerance improvement. Selecting stable reference genes for qRT-PCR analysis is critically important. The objective of this study was to determine the stability of expression for eight candidate reference genes (ACT, EF1a, TUB, UPL7, GAPDH, PP2A, PEPKR1, and CACS) in two tissues (roots and leaves) of a perennial grass species under four abiotic stresses (salt, drought, cold, and heat) using four programs (GeNorm, NormFinder, BestKeeper, and RefFinder). The results showed that (1) the combinations of CACS and UPL7 or PP2A and ACT were stably expressed in salt-treated roots or leaves; (2) the combinations of GAPDH and CACS or PP2A and PEPKR1 were stable in roots and leaves under drought stress; (3) CACS and PP2A exhibited stable expression in cold-treated roots and the combination of EF1a and UPL7 was also stable in cold-treated leaves; and (4) CACS and PP2A were the two most stable reference genes in heat-stressed roots and UPL7 combined with GAPDH and PP2A was stably expressed in heat-stressed leaves. The qRT-PCR analysis of a target gene, AsSAP expression patterns in response to salinity and drought stress, confirmed the reliability of those selected and stable reference genes. Identification of stable reference genes in creeping bentgrass will improve assay accuracy for selecting stress-tolerance genes and identifying molecular mechanisms conferring stress tolerance in this species.
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