ArticleDevelopmental dynamics : an official publication of the American Association of Anatomists2015
A medium-scale assay for enhancer validation in amniotes.
Article in Developmental dynamics : an official publication of the American Association of Anatomists, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- Targets of the transcription factor Six1 identify previously unreported candidate deafness genes.Development (Cambridge, England) · 2025Article
- Evolution of the expression and regulation of the nuclear hormone receptor ERR gene family in the chordate lineage.Developmental biology · 2023Article
- A gene regulatory network for neural induction.eLife · 2023Article
- Sox8 remodels the cranial ectoderm to generate the ear.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Enhancer activation by FGF signalling during otic induction.Developmental biology · 2020Article
- Gene, cell, and organ multiplication drives inner ear evolution.Developmental biology · 2017Review
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Authors and funding
2 authors.
Funding
Abstract
backgroundEnhancers are key elements to control gene expression in time and space and thus orchestrate gene function during development, homeostasis, and disease. Whole genome approaches and bioinformatic predictions have generated a tremendous pool of potential enhancers, however their spatiotemporal activity often remains to be validated in vivo. Despite recent progress in developing high throughput strategies for enhancer evaluation, these remain mainly restricted to invertebrates and in vitro cell culture.
resultsHere we design a medium-scale method to validate potential enhancers in an amniote embryo, the chick. Using a unique barcode for different reporter vectors allows us to detect the activity of nine separate enhancers in a single embryo by one-step RT-PCR. The assay is sufficiently sensitive to expand its capacity further by generating additional barcoded vectors.
conclusionsAs a rapid, sensitive, and cost-effective way to assess enhancer activity in an amniote vertebrate, this method provides a major advance and a useful alternative to the generation of transgenic animals.
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