Evidence map›Paper›PMID 26177184›Full record

ArticleIUCrJ2015

A novel inert crystal delivery medium for serial femtosecond crystallography.

Chelsie E Conrad, Shibom Basu, Daniel James, Dingjie Wang, Alexander Schaffer, Shatabdi Roy-Chowdhury, Nadia A Zatsepin, Andrew Aquila, Jesse Coe, Cornelius Gati and 14 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in IUCrJ, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.

0numbers the graph read from it
0cells of the map it votes in
68citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

68 citing papers in PubMed.

  1. Article
  2. Article
  3. Microfluidic Devices for High-Throughput In Situ Serial Crystallography.Advances in biochemical engineering/biotechnology · 2026
    Review
  4. Review
  5. Article
  6. Review
  7. Article
  8. Serial femtosecond crystallography.Nature reviews. Methods primers · 2022
    Article
  9. Article
  10. Ab initio reconstruction from one-dimensional crystal diffraction data.Acta crystallographica. Section A, Foundations and advances · 2022
    Article
  11. Article
  12. Review
  13. Article
  14. Article
  15. Article
  16. Article
  17. Article
  18. Article
  19. Dynamic Structural Biology Experiments at XFEL or Synchrotron Sources.Methods in molecular biology (Clifton, N.J.) · 2021
    Review
  20. Serial Crystallography for Structure-Based Drug Discovery.Trends in pharmacological sciences · 2020
    Review

8 more citing papers are in PubMed but not listed here.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

24 authors.

Chelsie E ConradDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.
Shibom BasuDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.
Daniel JamesCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
Dingjie WangDepartment of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
Alexander SchafferDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.
Shatabdi Roy-ChowdhuryDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.
Nadia A ZatsepinCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
Andrew AquilaSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.
Jesse CoeDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.
Cornelius GatiCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Mark S HunterSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.
Jason E KoglinSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.
Christopher KupitzCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, University of Wisconsin-Milwaukee , 1900 East Kenwood Boulevard, Milwaukee, WI 53211, USA.
Garrett NelsonCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
Ganesh SubramanianCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
Thomas A WhiteCenter for Free-Electron Laser Science, Deutsches Elektronen-Synchrotron DESY , Notkestrasse 85, 22607 Hamburg, Germany.
Yun ZhaoCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
James ZookDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.
Sébastien BoutetSLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA.
Vadim CherezovBridge Institute, Department of Chemistry, University of Southern California , 3430 S. Vermont Avenue, Los Angeles, CA 90089, USA.
John C H SpenceCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
Raimund FrommeDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.
Uwe WeierstallCenter for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA ; Department of Physics, Arizona State University , PO Box 871604, Tempe, AZ 85287-1504, USA.
Petra FrommeDepartment of Chemistry and Biochemistry, Arizona State University , PO Box 871604, Tempe, AZ 85287-1604, USA ; Center for Applied Structural Discovery, The Biodesign Institute , PO Box 875001, Tempe, AZ 85287-5001, USA.

Funding

Center for the Rational Design of Membrane Protein CrystallographyU54GM094599 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2014
$6.8M
Femtosecond nano-crystallography of membrane proteinsR01GM095583 · NIGMS · ARIZONA STATE UNIVERSITY-TEMPE CAMPUS · PI FROMME, PETRA · 2010 to 2023
$4.3M
Tracking conformational states in enzyme catalysts using correlated X-ray scatterR01GM097463 · NIGMS · STANFORD UNIVERSITY · PI DONIACH, SEBASTIAN · 2012 to 2015
$1.7M
HT structure determination of GPCRs by LCP serial femtosecond nanocrystallographyR01GM108635 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI CHEREZOV, VADIM · 2014 to 2017
$1.5M
NIGMS NIH HHS R01 GM095583NIGMS NIH HHS R01 GM097463NIGMS NIH HHS R01 GM108635NIGMS NIH HHS U54 GM094599
6 · The paper itself

Abstract

Serial femtosecond crystallography (SFX) has opened a new era in crystallo-graphy by permitting nearly damage-free, room-temperature structure determination of challenging proteins such as membrane proteins. In SFX, femtosecond X-ray free-electron laser pulses produce diffraction snapshots from nanocrystals and microcrystals delivered in a liquid jet, which leads to high protein consumption. A slow-moving stream of agarose has been developed as a new crystal delivery medium for SFX. It has low background scattering, is compatible with both soluble and membrane proteins, and can deliver the protein crystals at a wide range of temperatures down to 4°C. Using this crystal-laden agarose stream, the structure of a multi-subunit complex, phycocyanin, was solved to 2.5 Å resolution using 300 µg of microcrystals embedded into the agarose medium post-crystallization. The agarose delivery method reduces protein consumption by at least 100-fold and has the potential to be used for a diverse population of proteins, including membrane protein complexes.

Indexed as

coherent X-ray diffractive imagingfemtosecond studiesfree-electron lasermembrane proteinsnanocrystalsprotein complexesserial femtosecond crystallographyviscous crystal delivery

Identifiers

PMID26177184
PMCPMC4491314

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.