ArticleBiotechnology for biofuels2015
Butanol production in S. cerevisiae via a synthetic ABE pathway is enhanced by specific metabolic engineering and butanol resistance.
Article in Biotechnology for biofuels, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 50 citations in OpenAlex.
- Advances inJournal of fungi (Basel, Switzerland) · 2023Review
- Yeast Genomics and Its Applications in Biotechnological Processes: What Is Our Present and Near Future?Journal of fungi (Basel, Switzerland) · 2022Review
- Glucose/Xylose Co-FermentingFrontiers in bioengineering and biotechnology · 2022Article
- n-Butanol production by Saccharomyces cerevisiae from protein-rich agro-industrial by-products.Brazilian journal of microbiology : [publication of the Brazilian Society for Microbiology] · 2020Article
- Butanol production by Saccharomyces cerevisiae: perspectives, strategies and challenges.World journal of microbiology & biotechnology · 2020Review
- Anti-Contamination Strategies for Yeast Fermentations.Microorganisms · 2020Review
- Genotype-by-Environment-by-Environment Interactions in theG3 (Bethesda, Md.) · 2018Article
- n-Butanol production in S. cerevisiae: co-ordinate use of endogenous and exogenous pathways.Applied microbiology and biotechnology · 2018Article
- Evolutionary engineering improves tolerance for medium-chain alcohols inBiotechnology for biofuels · 2018Article
- Improving acetyl-CoA biosynthesis inBiotechnology for biofuels · 2017Article
- Lycopene overproduction in Saccharomyces cerevisiae through combining pathway engineering with host engineering.Microbial cell factories · 2016Article
- Metabolic engineering of a synergistic pathway for n-butanol production in Saccharomyces cerevisiae.Scientific reports · 2016Article
- n-Butanol production in Saccharomyces cerevisiae is limited by the availability of coenzyme A and cytosolic acetyl-CoA.Biotechnology for biofuels · 2016Article
- Increasing n-butanol production withBiotechnology for biofuels · 2016Article
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Authors and funding
8 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
backgroundThe fermentation of sugars to alcohols by microbial systems underpins many biofuel initiatives. Short chain alcohols, like n-butanol, isobutanol and isopropanol, offer significant advantages over ethanol in terms of fuel attributes. However, production of ethanol from resistant Saccharomyces cerevisiae strains is significantly less complicated than for these alternative alcohols.
resultsIn this study, we have transplanted an n-butanol synthesis pathway largely from Clostridial sp. to the genome of an S. cerevisiae strain. Production of n-butanol is only observed when additional genetic manipulations are made to restore any redox imbalance and to drive acetyl-CoA production. We have used this butanol production strain to address a key question regarding the sensitivity of cells to short chain alcohols. In the past, we have defined specific point mutations in the translation initiation factor eIF2B based upon phenotypic resistance/sensitivity to high concentrations of exogenously added n-butanol. Here, we show that even during endogenous butanol production, a butanol resistant strain generates more butanol than a butanol sensitive strain.
conclusionThese studies demonstrate that appreciable levels of n-butanol can be achieved in S. cerevisiae but that significant metabolic manipulation is required outside of the pathway converting acetyl-CoA to butanol. Furthermore, this work shows that the regulation of protein synthesis by short chain alcohols in yeast is a critical consideration if higher yields of these alcohols are to be attained.
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