ArticleFrontiers in plant science2015
Quantitative analysis of proteome extracted from barley crowns grown under different drought conditions.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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28 citing papers in PubMed, 66 citations in OpenAlex.
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- Drought-induced molecular changes in crown of various barley phytohormone mutants.Plant signaling & behavior · 2024Article
- Heat stress causes chromatin accessibility and related gene expression changes in crown tissues of barley (Hordeum vulgare).Plant molecular biology · 2024Article
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- Similar Transcriptomic Responses to Early and Late Drought Stresses Produce Divergent Phenotypes in Sunflower (International journal of molecular sciences · 2023Article
- High-throughput sequencing data revealed genotype-specific changes evoked by heat stress in crown tissue of barley sdw1 near-isogenic lines.BMC genomics · 2022Article
- Proteomic responses of two spring wheat cultivars to the combined water deficit and aphid (Frontiers in plant science · 2022Article
- Engineering Macromolecular Trafficking Into the Citrus Vasculature.Frontiers in plant science · 2022Article
- Application of Mass Spectrometry-Based Proteomics to Barley Research.Journal of agricultural and food chemistry · 2021Review
- Comparative proteomics analysis of Tibetan hull-less barley under osmotic stress via data-independent acquisition mass spectrometry.GigaScience · 2020Article
- Comparative Proteomic and Morpho-Physiological Analyses of Maize Wild-Type Vp16 and Mutant vp16 Germinating Seed Responses to PEG-Induced Drought Stress.International journal of molecular sciences · 2019Article
- Comparative proteomic study of phytotoxic effects of silver nanoparticles and silver ions on tobacco plants.Environmental science and pollution research international · 2019Article
- Breeding drought-resistant crops: G×E interactions, proteomics and pQTLS.Journal of experimental botany · 2019Article
- Identification of drought responsive proteins and related proteomic QTLs in barley.Journal of experimental botany · 2019Article
- Mitochondrial Biogenesis in Diverse Cauliflower Cultivars under Mild and Severe Drought. Impaired Coordination of Selected Transcript and Proteomic Responses, and Regulation of Various Multifunctional Proteins.International journal of molecular sciences · 2018Article
- Plant Abiotic Stress Proteomics: The Major Factors Determining Alterations in Cellular Proteome.Frontiers in plant science · 2018Review
- Evolutionary relationships and expression analysis of EUL domain proteins in rice (Oryza sativa).Rice (New York, N.Y.) · 2017Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Barley cultivar Amulet was used to study the quantitative proteome changes through different drought conditions utilizing two-dimensional difference gel electrophoresis (2D-DIGE). Plants were cultivated for 10 days under different drought conditions. To obtain control and differentially drought-treated plants, the soil water content was kept at 65, 35, and 30% of soil water capacity (SWC), respectively. Osmotic potential, water saturation deficit, (13)C discrimination, and dehydrin accumulation were monitored during sampling of the crowns for proteome analysis. Analysis of the 2D-DIGE gels revealed 105 differentially abundant spots; most were differentially abundant between the controls and drought-treated plants, and 25 spots displayed changes between both drought conditions. Seventy-six protein spots were successfully identified by tandem mass spectrometry. The most frequent functional categories of the identified proteins can be put into the groups of: stress-associated proteins, amino acid metabolism, carbohydrate metabolism, as well as DNA and RNA regulation and processing. Their possible role in the response of barley to drought stress is discussed. Our study has shown that under drought conditions barley cv. Amulet decreased its growth and developmental rates, displayed a shift from aerobic to anaerobic metabolism, and exhibited increased levels of several protective proteins. Comparison of the two drought treatments revealed plant acclimation to milder drought (35% SWC); but plant damage under more severe drought treatment (30% SWC). The results obtained revealed that cv. Amulet is sensitive to drought stress. Additionally, four spots revealing a continuous and significant increase with decreasing SWC (UDP-glucose 6-dehydrogenase, glutathione peroxidase, and two non-identified) could be good candidates for testing of their protein phenotyping capacity together with proteins that were significantly distinguished in both drought treatments.
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