ArticleFrontiers in plant science2015
Proteomic analysis of apoplastic fluid of Coffea arabica leaves highlights novel biomarkers for resistance against Hemileia vastatrix.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers, 1 of them a synthesis that pooled it.
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Who cites it
20 citing papers in PubMed, 1 synthesis or guideline pooled it, 47 citations in OpenAlex.
- The front line of defence: a meta-analysis of apoplastic proteases in plant immunity.Journal of experimental botany · 2021Pooled it
- Genome-Wide Analysis Reveals Chitinases as Putative Defense-Related Proteins Against Fungi in the Genomes ofPlants (Basel, Switzerland) · 2025Article
- Apoplastomes of contrasting cacao genotypes to witches' broom disease reveals differential accumulation of PR proteins.Frontiers in plant science · 2024Article
- The Underexplored Mechanisms of Wheat Resistance to Leaf Rust.Plants (Basel, Switzerland) · 2023Review
- Analysis of the apoplast fluid proteome during the induction of systemic acquired resistance inPeerJ · 2023Article
- CRISPR technology towards genome editing of the perennial and semi-perennial crops citrus, coffee and sugarcane.Frontiers in plant science · 2023Review
- Mapping of prehaustorial resistance against wheat leaf rust in einkorn (Frontiers in plant science · 2023Article
- Constitutive Defense Strategy of Coffee Under Field Conditions: A Comparative Assessment of Resistant and Susceptible Cultivars to Rust.Molecular biotechnology · 2022Article
- Leaf infiltration in plant science: old method, new possibilities.Plant methods · 2021Review
- Speaking the language of lipids: the cross-talk between plants and pathogens in defence and disease.Cellular and molecular life sciences : CMLS · 2021Review
- Elucidating the interactions between the rustiScience · 2021Article
- The Resistance of Oilseed Rape Microspore-Derived Embryos to Osmotic Stress Is Associated With the Accumulation of Energy Metabolism Proteins, Redox Homeostasis, Higher Abscisic Acid, and Cytokinin Contents.Frontiers in plant science · 2021Article
- The Apoplast: A Key Player in Plant Survival.Antioxidants (Basel, Switzerland) · 2020Review
- Primary Metabolism Is Distinctly Modulated by Plant Resistance Inducers inFrontiers in plant science · 2020Article
- Subtilisin-like proteases in plant defence: the past, the present and beyond.Molecular plant pathology · 2018Review
- High throughput transcriptome analysis of coffee reveals prehaustorial resistance in response to Hemileia vastatrix infection.Plant molecular biology · 2017Article
- The coffee leaf rust pathogen Hemileia vastatrix: one and a half centuries around the tropics.Molecular plant pathology · 2017Review
- Review
- Protein Dynamics in the Plant Extracellular Space.Proteomes · 2016Review
- Unconventional protein secretion in plants: a critical assessment.Protoplasma · 2016Review
Corrections and comments
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Authors and funding
10 authors at 3 institutions in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A proteomic analysis of the apoplastic fluid (APF) of coffee leaves was conducted to investigate the cellular processes associated with incompatible (resistant) and compatible (susceptible) Coffea arabica-Hemileia vastatrix interactions, during the 24-96 hai period. The APF proteins were extracted by leaf vacuum infiltration and protein profiles were obtained by 2-DE. The comparative analysis of the gels revealed 210 polypeptide spots whose volume changed in abundance between samples (control, resistant and susceptible) during the 24-96 hai period. The proteins identified were involved mainly in protein degradation, cell wall metabolism and stress/defense responses, most of them being hydrolases (around 70%), particularly sugar hydrolases and peptidases/proteases. The changes in the APF proteome along the infection process revealed two distinct phases of defense responses, an initial/basal one (24-48 hai) and a late/specific one (72-96 hai). Compared to susceptibility, resistance was associated with a higher number of proteins, which was more evident in the late/specific phase. Proteins involved in the resistance response were mainly, glycohydrolases of the cell wall, serine proteases and pathogen related-like proteins (PR-proteins), suggesting that some of these proteins could be putative candidates for resistant markers of coffee to H. vastatrix. Antibodies were produced against chitinase, pectin methylesterase, serine carboxypeptidase, reticuline oxidase and subtilase and by an immunodetection assay it was observed an increase of these proteins in the resistant sample. With this methodology we have identified proteins that are candidate markers of resistance and that will be useful in coffee breeding programs to assist in the selection of cultivars with resistance to H. vastatrix.
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