ArticleFrontiers in physiology2015
Sodium renders endothelial cells sticky for red blood cells.
Article in Frontiers in physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 44 citations in OpenAlex.
- Individual cell motion in healthy human skin microvasculature by reflectance confocal video microscopy.Microcirculation (New York, N.Y. : 1994) · 2020Trial
- Seeing What Sticks: Anchoring Capabilities of Moieties for Use in Cell-Conveyed Therapeutics.Bioconjugate chemistry · 2025Article
- Cellular and Molecular Resolution of Focal Segmental Glomerulosclerosis Recurrence in Human Allografts.bioRxiv : the preprint server for biology · 2025Article
- Shape-Dependent Structural Order of Red Blood Cells.Langmuir : the ACS journal of surfaces and colloids · 2025Article
- Bacterial Opsonization Changes Adhesion Interactions between Endothelial Cells and Neutrophils in a Model of Experimental Septicemia.Bulletin of experimental biology and medicine · 2024Article
- Erythrocyte glycocalyx sensitivity to sodium is associated with salt sensitivity of blood pressure in women but not men.Frontiers in nutrition · 2024Article
- Glycocalyx-Sodium Interaction in Vascular Endothelium.Nutrients · 2023Review
- Erythrocytes Functionality in SARS-CoV-2 Infection: Potential Link with Alzheimer's Disease.International journal of molecular sciences · 2023Review
- Article
- Red Blood Cells: Tethering, Vesiculation, and Disease in Micro-Vascular Flow.Diagnostics (Basel, Switzerland) · 2021Article
- Red Blood Cell Stiffness and Adhesion Are Species-Specific Properties Strongly Affected by Temperature and Medium Changes in Single Cell Force Spectroscopy.Molecules (Basel, Switzerland) · 2021Article
- Dynamic AFM detection of the oxidation-induced changes in size, stiffness, and stickiness of low-density lipoprotein.Journal of nanobiotechnology · 2020Article
- Endothelial Nanomechanics in the Context of Endothelial (Dys)function and Inflammation.Antioxidants & redox signaling · 2019Review
- Reducing Salt Intake and Exercising Regularly: Implications From Molecular Dynamics Simulations of Endothelial Glycocalyx.Frontiers in physiology · 2018Article
- KOncotarget · 2017Article
- Nanomechanics of the endothelial glycocalyx contribute to NaScientific reports · 2017Article
- Extracellular protonation modulates cell-cell interaction mechanics and tissue invasion in human melanoma cells.Scientific reports · 2017Article
- High sodium diet converts renal proteoglycans into pro-inflammatory mediators in rats.PloS one · 2017Article
- Cyp2c44 gene disruption exacerbated pulmonary hypertension and heart failure in female but not male mice.Pulmonary circulation · 2016Article
- Disturbed flow mediated modulation of shear forces on endothelial plane: A proposed model for studying endothelium around atherosclerotic plaques.Scientific reports · 2016Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Negative charges in the glycocalyx of red blood cells (RBC) and vascular endothelial cells (EC) facilitate frictionless blood flow through blood vessels. Na(+) selectively shields these charges controlling surface electronegativity. The question was addressed whether the ambient Na(+) concentration controls RBC-EC interaction. Using atomic force microscopy (AFM) adhesion forces between RBC and endothelial glycocalyx were quantified. A single RBC, mounted on an AFM cantilever, was brought in physical contact with the endothelial surface and then pulled off. Adhesion forces were quantified (i) after enzymatic removal of negative charges in the glycocalyx, (ii) under different ambient Na(+) and (iii) after applying the intracellular aldosterone receptor antagonist spironolactone. Removal of negative surface charges increases RBC-EC interaction forces. A stepwise increase of ambient Na(+) from 133 to 140 mM does not affect them. However, beyond 140 mM Na(+) adhesion forces increase sharply (10% increase of adhesion force per 1 mM increase of Na(+)). Spironolactone prevents this response. It is concluded that negative charges reduce adhesion between RBC and EC. Ambient Na(+) concentration determines the availability of free negative charges. Na(+) concentrations in the low physiological range (below 140 mM) allow sufficient amounts of vacant negative charges so that adhesion of RBC to the endothelial surface is small. In contrast, Na(+) in the high physiological range (beyond 140 mM) saturates the remaining negative surface charges thus increasing adhesion. Aldosterone receptor blockade by spironolactone prevents Na(+) induced RBC adhesion to the endothelial glycocalyx. Extrapolation of in vitro experiments to in vivo conditions leads to the hypothesis that high sodium intake is likely to increase the incidence of thrombotic events.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.