ArticleBreeding science2015
S-genotype identification based on allele-specific PCR in Japanese pear.
Article in Breeding science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 7 papers.
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Who cites it
7 citing papers in PubMed.
- A self-compatible pear mutant derived from γ-irradiated pollen carries an 11-Mb duplication in chromosome 17.Frontiers in plant science · 2024Article
- A semi in vivo pollination technique to assess the level of gametophytic self-incompatibility and pollen tube growth in pear (Pyrus communis L.).Plant reproduction · 2022Article
- Promising Perspectives for Detection, Identification, and Quantification of Plant Pathogenic Fungi and Oomycetes through Targeting Mitochondrial DNA.International journal of molecular sciences · 2020Review
- Self-(In)compatibility Systems: Target Traits for Crop-Production, Plant Breeding, and Biotechnology.Frontiers in plant science · 2020Review
- Rapid identification of apple (Plant biotechnology (Tokyo, Japan) · 2017Article
- Advances in Japanese pear breeding in Japan.Breeding science · 2016Review
- Genomics of pear and other Rosaceae fruit trees.Breeding science · 2016Review
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Gametophytic self-incompatibility in Japanese pear (Pyrus pyrifolia Nakai) is controlled by the single, multi-allelic S-locus. Information about the S-genotypes is important for breeding and the selection of pollen donors for fruit production. Rapid and reliable S-genotype identification system is necessary for efficient breeding of new cultivars in Japanese pear. We designed S allele-specific PCR primer pairs for ten previously reported S-RNase alleles (S (1)-S (9) and S (k)) as simple and reliable method. Specific nucleotide sequences were chosen to design the primers to amplify fragments of only the corresponding S alleles. The developed primer pairs were evaluated by using homozygous S-genotypes (S (1)/S (1)-S (9)/S (9) and S (4sm)/S (4sm)) and 14 major Japanese pear cultivars, and found that S allele-specific primer pairs can identify S-genotypes effectively. The S allele-specific primer pairs developed in this study will be useful for efficient S-genotyping and for marker-assisted selection in Japanese pear breeding programs.
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