ArticlePlant physiology2015
Identification of Open Stomata1-Interacting Proteins Reveals Interactions with Sucrose Non-fermenting1-Related Protein Kinases2 and with Type 2A Protein Phosphatases That Function in Abscisic Acid Responses.
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In one paragraph
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers, 1 of them a synthesis that pooled it.
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49citing papers in PubMed, 1 pooled it
14.0field-weighted citation impact, top 1% of its field
What it found
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Who cites it
49 citing papers in PubMed, 1 synthesis or guideline pooled it, 90 citations in OpenAlex.
- Dry side of the core: a meta-analysis addressing the original nature of the ABA signalosome at the onset of seed imbibition.Frontiers in plant science · 2023Pooled it
- A current view on the involvement of protein phosphatase PP2A subunits in the responses of plants to abiotic stress at the subcellular level.Plant cell reports · 2026Review
- Emerging concepts of reactive oxygen species functions in plants.Biochemical Society transactions · 2026Review
- Roles of ROS and NO in Plant Responses to Individual and Combined Salt Stress and Waterlogging.Antioxidants (Basel, Switzerland) · 2025Review
- Proteomics revealed novel functions and drought tolerance of Arabidopsis thaliana protein kinase ATG1.BMC biology · 2025Article
- Stomatal opening under high temperatures is controlled by the OST1-regulated TOT3-AHA1 module.Nature plants · 2025Article
- Extracellular niche establishment by plant pathogens.Nature reviews. Microbiology · 2024Review
- Differential metabolic reprogramming in developing soybean embryos in response to nutritional conditions and abscisic acid.Plant molecular biology · 2023Article
- Arabidopsis PLANT U-BOX44 down-regulates osmotic stress signaling by mediating Ca2+-DEPENDENT PROTEIN KINASE4 degradation.The Plant cell · 2023Article
- Distinct Clades of Protein Phosphatase 2A Regulatory B'/B56 Subunits Engage in Different Physiological Processes.International journal of molecular sciences · 2023Review
- ALA reverses ABA-induced stomatal closure by modulating PP2AC and SnRK2.6 activity in apple leaves.Horticulture research · 2023Article
- "B" Regulatory Subunits of PP2A: Their Roles in Plant Development and Stress Reactions.International journal of molecular sciences · 2023Review
- Phosphorylation status of Bβ subunit acts as a switch to regulate the function of phosphatase PP2A in ethylene-mediated root growth inhibition.The New phytologist · 2022Article
- Regulatory Network of Serine/Arginine-Rich (SR) Proteins: The Molecular Mechanism and Physiological Function in Plants.International journal of molecular sciences · 2022Review
- Overexpression ofInternational journal of molecular sciences · 2022Article
- Disentangling leaf-microbiome interactions inFrontiers in plant science · 2022Article
- Ethephon-Induced Ethylene Enhances Starch Degradation and Sucrose Transport with an Interactive Abscisic Acid-Mediated Manner in Mature Leaves ofPlants (Basel, Switzerland) · 2021Article
- The membrane-localized protein kinase MAP4K4/TOT3 regulates thermomorphogenesis.Nature communications · 2021Article
- Control of ABA Signaling and Crosstalk with Other Hormones by the Selective Degradation of Pathway Components.International journal of molecular sciences · 2021Review
- SPEECHLESS and MUTE Mediate Feedback Regulation of Signal Transduction during Stomatal Development.Plants (Basel, Switzerland) · 2021Review
Corrections and comments
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Authors and funding
14 authors at 3 institutions in 3 countries.
Rainer WaadtDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Bianca ManalansanDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Navin RauniyarDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).ORCID http://orcid.org/0000-0002-5297-7097
Shintaro MunemasaDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Matthew A BookerDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Benjamin BrandtDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Christian WaadtDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Dmitri A NusinowDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).ORCID http://orcid.org/0000-0002-0497-1723
Steve A KayDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Hans-Henning KunzDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).ORCID http://orcid.org/0000-0001-8000-0817
Karin SchumacherDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
Alison DeLongDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).
John R YatesDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.).ORCID http://orcid.org/0000-0001-5267-1672
Julian I SchroederDivision of Biological Sciences, Cell and Developmental Biology Section, and Center for Food and Fuel for the 21st Century, University of California, San Diego, La Jolla, California 92093-0116 (R.W., B.M., S.M., B.B., H.-H.K., J.I.S.);Centre for Organismal Studies, Plant Developmental Biology, University of Heidelberg, 69120 Heidelberg, Germany (R.W., K.S.);Department of Chemical Physiology, The Scripps Research Institute, La Jolla, California 92037 (N.R., J.R.Y.);Division of Agricultural and Life Science, Graduate School of Environmental and Life Science, Okayama University, Okayama 7008530, Japan (S.M.);Department of Molecular Biology, Cell Biology, and Biochemistry, Brown University, Providence, Rhode Island 02912 (M.A.B., A.D.);Department of Biology, Donald Danforth Plant Science Center, St. Louis, Missouri 63132 (D.A.N.); andMolecular and Computational Biology Section, University of Southern California, Los Angeles, California 90089 (S.A.K.) jischroeder@ucsd.edu.
Scripps Research Institute · USUniversity of California San Diego · USUniversity of Southern California · US
Funding
TrainingP41GM103533 · NIGMS · UNIVERSITY OF WASHINGTON · PI MACCOSS, MICHAEL · 2012 to 2021
$20.7MSTRESS HORMONE SIGNAL TRANSDUCTION IN ARABIDOPSISR01GM060396 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI SCHROEDER, JULIAN I · 2000 to 2023
$7.4MTRAINING IN MOLECULAR &CELL BIOLOGY &BIOCHEMISTRYT32GM007601 · NIGMS · BROWN UNIVERSITY · PI MOWRY, KIMBERLY L. · 1985 to 2019
$6.4MGENETIC ANALYSIS OF CIRCADIAN RHYTHMS IN ARABIDOPSISR01GM056006 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KAY, STEVE A, PRUNEDA-PAZ, JOSE L · 1996 to 2016
$6.0MMolecular Mechanisms in the Arabidopsis Circadian ClockR01GM067837 · NIGMS · UNIVERSITY OF SOUTHERN CALIFORNIA · PI KAY, STEVE A · 2003 to 2015
$4.7MDissection of EARLY FLOWERING3 Function in the Arabidopsis Circadian ClockF32GM083585 · NIGMS · UNIVERSITY OF CALIFORNIA, SAN DIEGO · PI NUSINOW, DMITRI ANTON · 2008 to 2010
$149kNIGMS NIH HHS F32 GM083585NIGMS NIH HHS F32GM083585NIGMS NIH HHS GM060396NIGMS NIH HHS P41 GM103533NIGMS NIH HHS R01 GM056006NIGMS NIH HHS R01GM056006NIGMS NIH HHS R01 GM060396NIGMS NIH HHS R01 GM067837NIGMS NIH HHS R01GM067837NIGMS NIH HHS T32 GM007601
Abstract
The plant hormone abscisic acid (ABA) controls growth and development and regulates plant water status through an established signaling pathway. In the presence of ABA, pyrabactin resistance/regulatory component of ABA receptor proteins inhibit type 2C protein phosphatases (PP2Cs). This, in turn, enables the activation of Sucrose Nonfermenting1-Related Protein Kinases2 (SnRK2). Open Stomata1 (OST1)/SnRK2.6/SRK2E is a major SnRK2-type protein kinase responsible for mediating ABA responses. Arabidopsis (Arabidopsis thaliana) expressing an epitope-tagged OST1 in the recessive ost1-3 mutant background was used for the copurification and identification of OST1-interacting proteins after osmotic stress and ABA treatments. These analyses, which were confirmed using bimolecular fluorescence complementation and coimmunoprecipitation, unexpectedly revealed homo- and heteromerization of OST1 with SnRK2.2, SnRK2.3, OST1, and SnRK2.8. Furthermore, several OST1-complexed proteins were identified as type 2A protein phosphatase (PP2A) subunits and as proteins involved in lipid and galactolipid metabolism. More detailed analyses suggested an interaction network between ABA-activated SnRK2-type protein kinases and several PP2A-type protein phosphatase regulatory subunits. pp2a double mutants exhibited a reduced sensitivity to ABA during seed germination and stomatal closure and an enhanced ABA sensitivity in root growth regulation. These analyses add PP2A-type protein phosphatases as another class of protein phosphatases to the interaction network of SnRK2-type protein kinases.
Indexed as
Abscisic AcidArabidopsis ProteinsFluorescenceGerminationImmunoprecipitationMutationPlants, Genetically ModifiedProtein BindingProtein Interaction MapsProtein KinasesProtein MultimerizationProtein Phosphatase 2Protein Serine-Threonine KinasesProtein SubunitsReproducibility of ResultsTwo-Hybrid System TechniquesAbscisic AcidArabidopsis ProteinsOST1 protein, ArabidopsisProtein KinasesProtein Phosphatase 2Protein Serine-Threonine KinasesProtein SubunitsSnRK2 protein, Arabidopsis
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