ArticleG3 (Bethesda, Md.)2015
An Updated Collection of Sequence Barcoded Temperature-Sensitive Alleles of Yeast Essential Genes.
Article in G3 (Bethesda, Md.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
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Who cites it
33 citing papers in PubMed, 59 citations in OpenAlex.
- The power of many: when genetics met yeasts and high-throughput.Biological reviews of the Cambridge Philosophical Society · 2026Review
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- A screen for synthetic genetic interactions with the Saccharomyces cerevisiae hrq1ΔN allele.G3 (Bethesda, Md.) · 2025Article
- The highly conserved intron of tyrosine tRNA is critical forProceedings of the National Academy of Sciences of the United States of America · 2025Article
- A proteome-wide yeast degron collection for the dynamic study of protein function.The Journal of cell biology · 2025Article
- Dynamics of DNA damage-induced nuclear inclusions are regulated by SUMOylation of Btn2.Nature communications · 2024Article
- From beer to breadboards: yeast as a force for biological innovation.Genome biology · 2024Review
- Mapping temperature-sensitive mutations at a genome scale to engineer growth switches in Escherichia coli.Molecular systems biology · 2023Article
- The ER calcium channel Csg2 integrates sphingolipid metabolism with autophagy.Nature communications · 2023Article
- An unknown essential function of tRNA splicing endonuclease is linked to the integrated stress response and intron debranching.Genetics · 2023Article
- A Suite of New Strain Construction Vectors for Gene Expression Knockdown in Budding Yeast.ACS synthetic biology · 2023Article
- Genetic background and mistranslation frequency determine the impact of mistranslating tRNASerUGG.G3 (Bethesda, Md.) · 2022Article
- Ribosomal RNA 2'-Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- High-throughput replica-pinning approach to screen for yeast genes controlling low-frequency events.STAR protocols · 2022Article
- A deep dive into the RecQ interactome: something old and something new.Current genetics · 2021Review
- The amino acid substitution affects cellular response to mistranslation.G3 (Bethesda, Md.) · 2021Article
- Chemical rescue of mutant proteins in living Saccharomyces cerevisiae cells by naturally occurring small molecules.G3 (Bethesda, Md.) · 2021Article
- Environmental robustness of the global yeast genetic interaction network.Science (New York, N.Y.) · 2021Article
- A conformational switch driven by phosphorylation regulates the activity of the evolutionarily conserved SNARE Ykt6.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- The Genetic and Physical Interactomes of theG3 (Bethesda, Md.) · 2020Article
Corrections and comments
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
Systematic analyses of essential gene function using mutant collections in Saccharomyces cerevisiae have been conducted using collections of heterozygous diploids, promoter shut-off alleles, through alleles with destabilized mRNA, destabilized protein, or bearing mutations that lead to a temperature-sensitive (ts) phenotype. We previously described a method for construction of barcoded ts alleles in a systematic fashion. Here we report the completion of this collection of alleles covering 600 essential yeast genes. This resource covers a larger gene repertoire than previous collections and provides a complementary set of strains suitable for single gene and genomic analyses. We use deep sequencing to characterize the amino acid changes leading to the ts phenotype in half of the alleles. We also use high-throughput approaches to describe the relative ts behavior of the alleles. Finally, we demonstrate the experimental usefulness of the collection in a high-content, functional genomic screen for ts alleles that increase spontaneous P-body formation. By increasing the number of alleles and improving the annotation, this ts collection will serve as a community resource for probing new aspects of biology for essential yeast genes.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.