ArticleMethods in cell biology2015
Correlative light and electron microscopy analysis of the centrosome: A step-by-step protocol.
Article in Methods in cell biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
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Who cites it
8 citing papers in PubMed.
- Profiling the male germline genome to unravel its reproductive potential.Fertility and sterility · 2023Article
- TRIM37 prevents formation of condensate-organized ectopic spindle poles to ensure mitotic fidelity.The Journal of cell biology · 2021Article
- Practical method for superresolution imaging of primary cilia and centrioles by expansion microscopy using an amplibody for fluorescence signal amplification.Molecular biology of the cell · 2020Article
- With Age Comes Maturity: Biochemical and Structural Transformation of a Human Centriole in the Making.Cells · 2020Review
- High-resolution characterization of centriole distal appendage morphology and dynamics by correlative STORM and electron microscopy.Nature communications · 2019Article
- PLK4 is a microtubule-associated protein that self-assembles promotingJournal of cell science · 2018Article
- Separation and Loss of Centrioles From Primordidal Germ Cells To Mature Oocytes In The Mouse.Scientific reports · 2018Article
- Centriole triplet microtubules are required for stable centriole formation and inheritance in human cells.eLife · 2017Article
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Authors and funding
2 authors.
Funding
Abstract
Correlative light and electron microscopy harnesses the best from each of the two modalities of microscopy it utilizes; while light microscopy provides information about the dynamic properties of the cellular structure or fluorescently labeled protein, electron microscopy provides ultrastructural information in an unsurpassed resolution. However, tracing a particular cell and its rare and small structures such as centrosomes throughout numerous steps of the experiment is not a trivial task. In this chapter, we present the experimental workflow for combining live-cell fluorescence microscopy analysis with classical transmission electron microscopy, adapted for the studies of the centrosomes and basal bodies. We describe, in a step-by-step manner, an approach that can be affordably and successfully employed in any typical cell biology laboratory. The article details all key phases of the analysis starting from cell culture, live-cell microscopy, and sample fixation, through the steps of sample preparation for electron microscopy, to the identification of the target cell on the electron microscope.
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