Evidence map›Paper›PMID 26175208›Full record

ArticleMicrobiologyOpen2015

Functional expression, purification, and biochemical properties of subtilase SprP from Pseudomonas aeruginosa.

Alexander Pelzer, Christian Schwarz, Andreas Knapp, Astrid Wirtz, Susanne Wilhelm, Sander Smits, Lutz Schmitt, Horst Funken, Karl-Erich Jaeger

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in MicrobiologyOpen, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
0.3field-weighted citation impact, top 39% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed, 4 citations in OpenAlex.

  1. Dual Transcriptomics of Host-Pathogen Interaction of Cystic Fibrosis IsolateFrontiers in cellular and infection microbiology · 2018
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 1 institution in 1 country.

Alexander PelzerInstitute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Forschungszentrum Juelich, D-52426, Juelich, Germany.
Christian SchwarzInstitute of Biochemistry, Heinrich-Heine-University Duesseldorf, Universitaetsstr. 1, D-40225, Duesseldorf, Germany.
Andreas KnappInstitute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Forschungszentrum Juelich, D-52426, Juelich, Germany.
Astrid WirtzInstitute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Forschungszentrum Juelich, D-52426, Juelich, Germany.
Susanne WilhelmInstitute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Forschungszentrum Juelich, D-52426, Juelich, Germany.
Sander SmitsInstitute of Biochemistry, Heinrich-Heine-University Duesseldorf, Universitaetsstr. 1, D-40225, Duesseldorf, Germany.
Lutz SchmittInstitute of Biochemistry, Heinrich-Heine-University Duesseldorf, Universitaetsstr. 1, D-40225, Duesseldorf, Germany.
Horst FunkenInstitute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Forschungszentrum Juelich, D-52426, Juelich, Germany.
Karl-Erich JaegerInstitute of Molecular Enzyme Technology, Heinrich-Heine-University Duesseldorf, Forschungszentrum Juelich, D-52426, Juelich, Germany.
Heinrich Heine University Düsseldorf · DE

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The Pseudomonas aeruginosa genome encodes a variety of different proteolytic enzymes several of which play an important role as virulence factors. Interestingly, only two of these proteases are predicted to belong to the subtilase family and we have recently studied the physiological role of the subtilase SprP. Here, we describe the functional overexpression of SprP in Escherichia coli using a novel expression and secretion system. We show that SprP is autocatalytically activated by proteolysis and exhibits optimal activity at 50°C in a pH range of 7-8. We also demonstrate a significant increase in sprP promoter activity upon growth of P. aeruginosa at 43°C indicating a role for SprP in heat shock response.

Indexed as

Cloning, MolecularEnzyme StabilityEscherichia coliGene ExpressionGene Expression ProfilingHydrogen-Ion ConcentrationProteolysisPseudomonas aeruginosaRecombinant ProteinsSubtilisinsTemperatureRecombinant ProteinsSubtilisinsAutocatalytic processingheat inductionPseudomonas aeruginosasubtilase SprP.

Identifiers

PMID26175208
PMCPMC4618607
OpenAlexW1963282235

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.