Evidence map›Paper›PMID 26174828›Full record

ArticleThe Korean journal of parasitology2015

Cloning and Iron Transportation of Nucleotide Binding Domain of Cryptosporidium andersoni ATP-Binding Cassette (CaABC) Gene.

Ju-Hua Wang, Xiu-Heng Xue, Jie Zhou, Cai-Yun Fan, Qian-Qian Xie, Pan Wang

Open access · diamondAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in The Korean journal of parasitology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed, 0 citations in OpenAlex.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 1 institution in 1 country.

Ju-Hua WangCollege of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
Xiu-Heng XueCollege of Tea & Food Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
Jie ZhouCollege of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
Cai-Yun FanCollege of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
Qian-Qian XieCollege of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
Pan WangCollege of Animal Science and Technology, Anhui Agriculture University, Hefei, Anhui, 230036, China.
Anhui Agricultural University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Cryptosporidium andersoni ATP-binding cassette (CaABC) is an important membrane protein involved in substrate transport across the membrane. In this research, the nucleotide binding domain (NBD) of CaABC gene was amplified by PCR, and the eukaryotic expression vector of pEGFP-C1-CaNBD was reconstructed. Then, the recombinant plasmid of pEGFP-C1-CaNBD was transformed into the mouse intestinal epithelial cells (IECs) to study the iron transportation function of CaABC. The results indicated that NBD region of CaABC gene can significantly elevate the transport efficiency of Ca(2+), Mg(2+), K(+), and HCO3 (-) in IECs (P<0.05). The significance of this study is to find the ATPase inhibitors for NBD region of CaABC gene and to inhibit ATP binding and nutrient transport of CaABC transporter. Thus, C. andersoni will be killed by inhibition of nutrient uptake. This will open up a new way for treatment of cryptosporidiosis.

Indexed as

Cloning, MolecularAdenosine TriphosphateAmino Acid SequenceAnimalsATP-Binding Cassette TransportersCalciumCryptosporidiosisCryptosporidiumHumansIronMiceMolecular Sequence DataProtein Structure, TertiaryProtozoan ProteinsSequence AlignmentAdenosine TriphosphateATP-Binding Cassette TransportersCalciumIronProtozoan ProteinsATP-binding cassette transporter proteincloningCryptosporidium andersoniiron transportation

Identifiers

PMID26174828
PMCPMC4510675
OpenAlexW1917248883

What OpenQuestion holds

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LicenceCC BY-NC
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.