ArticleScientific reports2015
Polymerase/DNA interactions and enzymatic activity: multi-parameter analysis with electro-switchable biosurfaces.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Substrate energetics in non-templated DNA synthesis by terminal deoxynucleotidyl transferase.Nucleic acids research · 2026Article
- Cryptic DNA sequences encode a temperature sensor to regulate mitochondrial suicide during neuronal differentiation.Cell communication and signaling : CCS · 2026Article
- Mathematical Modeling and Thermodynamic Integration for Precision PCR Optimization.Iranian journal of biotechnology · 2025Article
- Electro-switchable addressing system for achieving repetitive random data access.Nucleic acids research · 2025Article
- Design and Optimization of Isothermal Gene Amplification for Generation of High-Gain Oligonucleotide Products by MicroRNAs.ACS measurement science au · 2024Article
- Reverse transcription as key step in RNARSC chemical biology · 2024Article
- Application of the SwitchSense Technique for the Study of Small Molecules' (Ethidium Bromide and Selected Sulfonamide Derivatives) Affinity to DNA in Real Time.The journal of physical chemistry. B · 2022Article
- Single-molecule Taq DNA polymerase dynamics.Science advances · 2022Article
- What Can Electrochemical Methods Offer in Determining DNA-Drug Interactions?Molecules (Basel, Switzerland) · 2021Review
- Signaling pathways have an inherent need for noise to acquire information.BMC bioinformatics · 2020Article
- DNA synthesis from diphosphate substrates by DNA polymerases.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Recent progress in dissecting molecular recognition by DNA polymerases with non-native substrates.Current opinion in chemical biology · 2017Review
- Mapping the sugar dependency for rational generation of a DNA-RNA hybrid-guided Cas9 endonuclease.Nature communications · 2017Article
- Structural study of the Fox-1 RRM protein hydration reveals a role for key water molecules in RRM-RNA recognition.Nucleic acids research · 2017Article
- Label-free optical detection of single enzyme-reactant reactions and associated conformational changes.Science advances · 2017Article
- Mathematics analysis of polymerase chain reaction kinetic curves.Doklady. Biochemistry and biophysics · 2016Article
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Authors and funding
7 authors.
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No grant is acknowledged in the PubMed record.
Abstract
The engineering of high-performance enzymes for future sequencing and PCR technologies as well as the development of many anticancer drugs requires a detailed analysis of DNA/RNA synthesis processes. However, due to the complex molecular interplay involved, real-time methodologies have not been available to obtain comprehensive information on both binding parameters and enzymatic activities. Here we introduce a chip-based method to investigate polymerases and their interactions with nucleic acids, which employs an electrical actuation of DNA templates on microelectrodes. Two measurement modes track both the dynamics of the induced switching process and the DNA extension simultaneously to quantitate binding kinetics, dissociation constants and thermodynamic energies. The high sensitivity of the method reveals previously unidentified tight binding states for Taq and Pol I (KF) DNA polymerases. Furthermore, the incorporation of label-free nucleotides can be followed in real-time and changes in the DNA polymerase conformation (finger closing) during enzymatic activity are observable.
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