ArticleProtein science : a publication of the Protein Society2015
Structure of a designed tetrahedral protein assembly variant engineered to have improved soluble expression.
Article in Protein science : a publication of the Protein Society, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed.
- I53-50: Engineered icosahedral protein cage for modular vaccine nanoplatform.Journal of microbiology (Seoul, Korea) · 2026Review
- Priming antibody responses to the fusion peptide in rhesus macaques.NPJ vaccines · 2024Article
- Modeling reveals the strength of weak interactions in stacked-ring assembly.Biophysical journal · 2024Article
- Accurate computational design of three-dimensional protein crystals.Nature materials · 2023Article
- Tailored Functionalized Protein Nanocarriers for Cancer Therapy: Recent Developments and Prospects.Pharmaceutics · 2023Review
- Improved interface packing and design opportunities revealed by CryoEM analysis of a designed protein nanocage.Heliyon · 2022Article
- Antigen- and scaffold-specific antibody responses to protein nanoparticle immunogens.Cell reports. Medicine · 2022Article
- Protein Assembly by Design.Chemical reviews · 2021Review
- Polyclonal antibody responses to HIV Env immunogens resolved using cryoEM.Nature communications · 2021Article
- A fragment-based protein interface design algorithm for symmetric assemblies.Protein engineering, design & selection : PEDS · 2021Article
- Design and structure of two new protein cages illustrate successes and ongoing challenges in protein engineering.Protein science : a publication of the Protein Society · 2020Article
- Near-atomic cryo-EM imaging of a small protein displayed on a designed scaffolding system.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- The 2017 Keystone Symposium on HIV Vaccines.Human vaccines & immunotherapeutics · 2017Article
- Self-Assembly of an α-Helical Peptide into a Crystalline Two-Dimensional Nanoporous Framework.Journal of the American Chemical Society · 2016Article
- Accurate design of megadalton-scale two-component icosahedral protein complexes.Science (New York, N.Y.) · 2016Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
Abstract
We recently reported the development of a computational method for the design of coassembling multicomponent protein nanomaterials. While four such materials were validated at high-resolution by X-ray crystallography, low yield of soluble protein prevented X-ray structure determination of a fifth designed material, T33-09. Here we report the design and crystal structure of T33-31, a variant of T33-09 with improved soluble yield resulting from redesign efforts focused on mutating solvent-exposed side chains to charged amino acids. The structure is found to match the computational design model with atomic-level accuracy, providing further validation of the design approach and demonstrating a simple and potentially general means of improving the yield of designed protein nanomaterials.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.