Evidence map›Paper›PMID 26174131›Full record

ArticleScientific reports2015

Ultrasensitive measurement of huntingtin protein in cerebrospinal fluid demonstrates increase with Huntington disease stage and decrease following brain huntingtin suppression.

Amber L Southwell, Stephen E P Smith, Tessa R Davis, Nicholas S Caron, Erika B Villanueva, Yuanyun Xie, Jennifer A Collins, Min Li Ye, Aaron Sturrock, Blair R Leavitt and 2 more

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 57 papers.

0numbers the graph read from it
0cells of the map it votes in
57citing papers in PubMed
3.9field-weighted citation impact, top 5% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

57 citing papers in PubMed, 106 citations in OpenAlex.

  1. Trial
  2. High-affinity, structure-validated and selective macrocyclic peptide tools for chemical biology studies of Huntingtin.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  3. Article
  4. Article
  5. Article
  6. Review
  7. Article
  8. Article
  9. Review
  10. Article
  11. Review
  12. Article
  13. Article
  14. Article
  15. Article
  16. Review
  17. Article
  18. Review
  19. Article
  20. Fluid transport in the brain.Physiological reviews · 2022
    Review
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

12 authors at 3 institutions in 2 countries.

Amber L SouthwellCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Stephen E P SmithMayo Clinic College of Medicine, Department of Immunology, Rochester, MN 55905.
Tessa R DavisMayo Clinic College of Medicine, Department of Immunology, Rochester, MN 55905.
Nicholas S CaronCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Erika B VillanuevaCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Yuanyun XieCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Jennifer A CollinsCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Min Li YeCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Aaron SturrockCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Blair R LeavittCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Adam G SchrumMayo Clinic College of Medicine, Department of Immunology, Rochester, MN 55905.
Michael R HaydenCentre for Molecular Medicine and Therapeutics, Child and Family Research Institute, and Department of Medical Genetics, University of British Columbia, Vancouver, BC V5Z 4H4; Canada.
Child and Family Research Institute · CAMayo Clinic · USUniversity of British Columbia · CA

Funding

Measuring multiprotein assemblies that drive biological signalsR01GM103841 · NIGMS · UNIVERSITY OF MISSOURI-COLUMBIA · PI SCHRUM, ADAM G. · 2013 to 2023
$3.3M
Protein Interaction Network Analysis to Test the Synaptic Hypothesis of AutismK99MH102244 · NIMH · MAYO CLINIC ROCHESTER · PI SMITH, STEPHEN EDWARD PAUCHA · 2014 to 2015
$180k
Canadian Institutes of Health Research MOP-84438NIGMS NIH HHS R01 GM103841NIGMS NIH HHS R01GM103841NIMH NIH HHS K99 MH102244NIMH NIH HHS K99MH102244
6 · The paper itself

Abstract

Quantitation of huntingtin protein in the brain is needed, both as a marker of Huntington disease (HD) progression and for use in clinical gene silencing trials. Measurement of huntingtin in cerebrospinal fluid could be a biomarker of brain huntingtin, but traditional protein quantitation methods have failed to detect huntingtin in cerebrospinal fluid. Using micro-bead based immunoprecipitation and flow cytometry (IP-FCM), we have developed a highly sensitive mutant huntingtin detection assay. The sensitivity of huntingtin IP-FCM enables accurate detection of mutant huntingtin protein in the cerebrospinal fluid of HD patients and model mice, demonstrating that mutant huntingtin levels in cerebrospinal fluid reflect brain levels, increasing with disease stage and decreasing following brain huntingtin suppression. This technique has potential applications as a research tool and as a clinical biomarker.

Indexed as

AdultAgedAnimalsBrainDisease Models, AnimalFemaleFlow CytometryHumansHuntingtin ProteinHuntington DiseaseImmunoblottingImmunoprecipitationMaleMiceMiddle AgedMutationHTT protein, humanHuntingtin ProteinNerve Tissue ProteinsRecombinant Fusion Proteins

Identifiers

PMID26174131
PMCPMC4502413
OpenAlexW2271390580

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.