Evidence map›Paper›PMID 26174123›Full record

ArticleJournal of assisted reproduction and genetics2015

Phospholipase C-zeta deficiency as a cause for repetitive oocyte fertilization failure during ovarian stimulation for in vitro fertilization with ICSI: a case report.

Zahabiya H Chithiwala, Hoi Chang Lee, David L Hill, Teru Jellerette-Nolan, Rafael Fissore, Daniel Grow, Daniel A Dumesic

Open access · bronzeAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of assisted reproduction and genetics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
10citing papers in PubMed, 1 pooled it
1.7field-weighted citation impact, top 15% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

10 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.

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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors at 4 institutions in 1 country.

Zahabiya H ChithiwalaDepartment of Obstetrics and Gynecology, University of California Los Angeles, 10833 Le Conte Ave, Room 22-178 CHS, Los Angeles, CA, 90095, USA.
Hoi Chang LeeDepartment of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, 01003, USA.
David L HillART Reproductive Center, 450 North Roxbury Drive Suite 520, Beverly Hills, CA, 90210, USA.
Teru Jellerette-NolanDivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Baystate Medical Center, Western Campus of Tufts University School of Medicine, 280 Chestnut Street, Springfield, MA, 01199, USA.
Rafael FissoreDepartment of Veterinary and Animal Sciences, University of Massachusetts, Amherst, MA, 01003, USA.
Daniel GrowDivision of Reproductive Endocrinology, Department of Obstetrics and Gynecology, Baystate Medical Center, Western Campus of Tufts University School of Medicine, 280 Chestnut Street, Springfield, MA, 01199, USA.
Daniel A DumesicDepartment of Obstetrics and Gynecology, University of California Los Angeles, 10833 Le Conte Ave, Room 22-178 CHS, Los Angeles, CA, 90095, USA. danieldumesic@aol.com.
Baystate Medical Center · USUniversity of California, Los Angeles · USUniversity of Massachusetts Amherst · USART Reproductive Center · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeThe purpose of this study is to describe impaired oocyte fertilization from phospholipase C-zeta (PLC-ζ) deficiency in normal-appearing sperm that was successfully treated using calcium (Ca(2+)) ionophore with intracytoplasmic sperm injection (ICSI) of oocytes matured in vitro.

methodsAn infertile couple undergoing in vitro fertilization (IVF) experienced failed oocyte fertilization following ICSI with normal-appearing sperm. A semen sample collected from the patient was used to assess the expression of sperm PLC- ζ protein by Western blot analysis and immunofluorescence and PLC-ζ bioactivity by an in vitro model of Ca(2+) release. A second IVF cycle was performed using Ca(2+) ionophore with ICSI to enhance Ca(2+)-induced oocyte activation of oocytes matured in vitro.

resultsSperm PLC-ζ protein deficiency was demonstrated by Western blot analysis and immunofluorescence and confirmed by reduced PLC-ζ bioactivity using an in vitro model of Ca(2+) release. Nevertheless, with this sperm and supplementation of Ca(2+) ionophore following ICSI, fertilization of four of six oocytes matured in vitro was obtained. In addition, four embryos underwent cleavage and two of them reached the blastocyst stage. Transfer of these blastocysts into the uterus led to a single pregnancy and live birth.

conclusionsDeficiency of PLC-ζ in normal-appearing human sperm is associated with impaired Ca(2+)-dependent oocyte activation during ICSI. Under this condition, use of Ca(2+) ionophore following ICSI of oocytes matured in vitro improves embryo developmental competence, possibly through the activation of Ca(2+)-dependent mechanisms governing fertilization and preimplantation embryogenesis.

Indexed as

Ovulation InductionAdultBlotting, WesternEmbryo TransferFemaleFertilizationFluorescent Antibody TechniqueHumansInfant, NewbornInfertilityMaleOocytesPhosphoinositide Phospholipase CPregnancySpermatozoaSperm Injections, IntracytoplasmicPhosphoinositide Phospholipase CPLCZ1 protein, humanAssisted reproductive technology (ART)Calcium ionophoreIn vitro maturationOocyte activationPhospholipase C-zetaSperm

Identifiers

PMID26174123
PMCPMC4595401
OpenAlexW909688392

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.