ArticlePloS one2015
The Semen pH Affects Sperm Motility and Capacitation.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 54 papers, 3 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
54 citing papers in PubMed, 3 syntheses or guidelines pooled it, 157 citations in OpenAlex.
- Per capita sperm metabolism is density dependent.The Journal of experimental biology · 2024Pooled it
- Effects of nonoxynol-9 (N-9) on sperm functions: systematic review and meta-analysis.Reproduction & fertility · 2022Pooled it
- The impact of ejaculatory abstinence on semen analysis parameters: a systematic review.Journal of assisted reproduction and genetics · 2018Pooled it
- Optimization of Semen Extender for Mongolian Sheep by Integrating Metabolomics and Sperm Quality Analysis at 17 °C with Antioxidant Supplementation.Animals : an open access journal from MDPI · 2026Article
- First-in-line 3D-printed intravaginal ring for nonhormonal contraception.Drug delivery and translational research · 2026Article
- Article
- Semen characteristics of Uda rams supplemented with graded levels of Moringa oleifera leaf.Tropical animal health and production · 2026Article
- Longer Arousal, Better Semen: Effects of Extended Pre-Ejaculatory Arousal on Semen Parameters in Infertile Men.Diagnostics (Basel, Switzerland) · 2025Article
- First-in-line 3D-printed intravaginal ring for nonhormonal contraception.Research square · 2025Article
- Timing Matters? Impact of Early Sperm Processing on Motile Sperm Recovery, Fertilization, Blastocyst Rate, and Pregnancy Outcomes in IUI and IVF.Journal of clinical medicine · 2025Article
- A prodrug strategy for sustained release of lactic acid from silicone elastomer vaginal rings.International journal of pharmaceutics · 2025Article
- Characterisation of phosphate transport in epididymis and prostate with possible relevance for semen quality.EBioMedicine · 2025Article
- Bioresponsive Hydrogel for On-Demand Nonhormonal Contraception.Gels (Basel, Switzerland) · 2025Article
- Sperm Membrane Stability: In-Depth Analysis from Structural Basis to Functional Regulation.Veterinary sciences · 2025Review
- Factorial analysis of zinc serum levels, fatty acids, oxidative stress parameters and supplementation on assisted reproductive technology outcome.Journal of medical biochemistry · 2025Article
- Decoding a novel non-enzymatic protein acetylation mechanism in sperm that is essential for fertilizing potential.Biological research · 2025Article
- Non-hormonal Contraception: Current and Emerging Targets.Advances in experimental medicine and biology · 2025Review
- Effect of pH and neutrophil count on the motility and persistence of spermatozoa in the vagina of candidiasis rat models.Narra J · 2024Article
- Genetic Selection Approach for Semen Characteristics in Thai Native Grandparent Roosters (Pradu Hang Dum) Using Random Regression Test-Day Models and Selection Indices.Animals : an open access journal from MDPI · 2024Article
- pH Homeodynamics and Male Fertility: A Coordinated Regulation of Acid-Based Balance during Sperm Journey to Fertilization.Biomolecules · 2024Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
8 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
As the chemical environment of semen can have a profound effect on sperm quality, we examined the effect of pH on the motility, viability and capacitation of human sperm. The sperm in this study was collected from healthy males to avoid interference from other factors. The spermatozoa cultured in sperm nutrition solution at pH 5.2, 6.2, 7.2 and 8.2 were analyzed for sperm total motility, progressive motility (PR), hypo-osmotic swelling (HOS) rate, and sperm penetration. Our results showed that these parameters were similar in pH 7.2 and 8.2 sperm nutrition solutions, but decreased in pH 5.2 and 6.2 solutions. The HOS rate exhibited positive correlation with the sperm total motility and PR. In addition, the sperm Na(+)/K(+)-ATPase activity at different pHs was measured, and the enzyme activity was significantly lower in pH 5.2 and 6.2 media, comparing with that in pH 8.2 and pH 7.2 solutions. Using flow cytometry (FCM) and laser confocal scanning microscopy (LCSM) analysis, the intracellular Ca2(+ )concentrations of sperm cultured in sperm capacitation solution at pH 5.2, 6.2, 7.2 and 8.2 were determined. Compared with that at pH 7.2, the mean fluorescence intensity of sperm in pH 5.2 and 6.2 media decreased significantly, while that of pH 8.2 group showed no difference. Our results suggested that the declined Na(+)/K(+)-ATPase activity at acidic pHs result in decreased sperm movement and capacitation, which could be one of the mechanisms of male infertility.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.