ArticleThe Journal of physiology2015
Age-related differences in lean mass, protein synthesis and skeletal muscle markers of proteolysis after bed rest and exercise rehabilitation.
Article in The Journal of physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 113 papers, 4 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
113 citing papers in PubMed, 4 syntheses or guidelines pooled it, 218 citations in OpenAlex.
- Ergogenic Benefits of β-Hydroxy-β-Methyl Butyrate (HMB) Supplementation on Body Composition and Muscle Strength: An Umbrella Review of Meta-Analyses.Journal of cachexia, sarcopenia and muscle · 2025Pooled it
- The role of muscle disuse in muscular and cardiovascular fitness: A systematic review and meta-regression.European journal of sport science · 2024Pooled it
- Pooled it
- The Aging Muscle in Experimental Bed Rest: A Systematic Review and Meta-Analysis.Frontiers in nutrition · 2021Pooled it
- Leucine supplementation does not attenuate the decline in daily muscle protein synthesis rates or preserve leg muscle mass during leg immobilization in young or older adults: a double-blind randomized trial.The American journal of clinical nutrition · 2026Trial
- Does high frequency exercise or electrical muscle stimulation improve strength and function in non-weight bearing, older patients? The PAIVE pilot randomised trial.BMC geriatrics · 2025Trial
- Trial
- Nudge-Based Interventions on Health Promotion Activity Among Very Old People: A Pragmatic, 2-Arm, Participant-Blinded Randomized Controlled Trial.Journal of the American Medical Directors Association · 2023Trial
- Neuromuscular electrical stimulation and protein during bed rest increases CD11bApplied physiology, nutrition, and metabolism = Physiologie appliquee, nutrition et metabolisme · 2020Trial
- Metabolic Implications of Diet and Energy Intake during Physical Inactivity.Medicine and science in sports and exercise · 2019Trial
- Skeletal muscle ceramides and relationship with insulin sensitivity after 2 weeks of simulated sedentary behaviour and recovery in healthy older adults.The Journal of physiology · 2018Trial
- Trial
- Neuromuscular Electrical Stimulation Combined with Protein Ingestion Preserves Thigh Muscle Mass But Not Muscle Function in Healthy Older Adults During 5 Days of Bed Rest.Rejuvenation research · 2017Trial
- Musculoskeletal decay and bone-muscle crosstalk during physical disuse: Mechanisms and integrated countermeasures.Experimental physiology · 2026Review
- Unlocking high-intensity performance thresholds through ventilatory signatures in the ECG.Scientific reports · 2026Article
- Muscle atrophy after ACL reconstruction involves molecular mechanisms beyond unloading.Journal of applied physiology (Bethesda, Md. : 1985) · 2026Article
- Impact of 14 days of head-down bed rest and an exercise countermeasure on skeletal muscle atrophy, proteome and circulatory cytokines in older adults.Experimental physiology · 2026Article
- The effects of bed rest on cardiometabolic health: A systematic review and meta-analysis.Experimental physiology · 2026Article
- Exploring perspectives of older adults and informal caregivers on physical activity during non-weight-bearing rehabilitation: a qualitative study.European geriatric medicine · 2026Article
- Physical activity interventions to improve physical function in temporarily non-ambulant older persons: a scoping review.Frontiers in aging · 2026Review
53 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
Abstract
Bed rest-induced muscle loss and impaired muscle recovery may contribute to age-related sarcopenia. It is unknown if there are age-related differences in muscle mass and muscle anabolic and catabolic responses to bed rest. A secondary objective was to determine if rehabilitation could reverse bed rest responses. Nine older and fourteen young adults participated in a 5-day bed rest challenge (BED REST). This was followed by 8 weeks of high intensity resistance exercise (REHAB). Leg lean mass (via dual-energy X-ray absorptiometry; DXA) and strength were determined. Muscle biopsies were collected during a constant stable isotope infusion in the postabsorptive state and after essential amino acid (EAA) ingestion on three occasions: before (PRE), after bed rest and after rehabilitation. Samples were assessed for protein synthesis, mTORC1 signalling, REDD1/2 expression and molecular markers related to muscle proteolysis (MURF1, MAFBX, AMPKα, LC3II/I, Beclin1). We found that leg lean mass and strength decreased in older but not younger adults after bedrest (P < 0.05) and was restored after rehabilitation. EAA-induced mTORC1 signalling and protein synthesis increased before bed rest in both age groups (P < 0.05). Although both groups had blunted mTORC1 signalling, increased REDD2 and MURF1 mRNA after bedrest, only older adults had reduced EAA-induced protein synthesis rates and increased MAFBX mRNA, p-AMPKα and the LC3II/I ratio (P < 0.05). We conclude that older adults are more susceptible than young persons to muscle loss after short-term bed rest. This may be partially explained by a combined suppression of protein synthesis and a marginal increase in proteolytic markers. Finally, rehabilitation restored bed rest-induced deficits in lean mass and strength in older adults.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.