ArticleThe ISME journal2016
Linking metabolite production to taxonomic identity in environmental samples by (MA)LDI-FISH.
Article in The ISME journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed.
- Omics for deciphering oral microecology.International journal of oral science · 2024Review
- Visualization of metabolites and microbes at high spatial resolution using MALDI mass spectrometry imaging and in situ fluorescence labeling.Nature protocols · 2023Review
- Bacterial ectosymbionts in cuticular organs chemically protect a beetle during molting stages.The ISME journal · 2022Article
- The Exploration of Microbial Natural Products and Metabolic Interaction Guided by Mass Spectrometry Imaging.Bioengineering (Basel, Switzerland) · 2022Review
- Recent advances in tools to map the microbiome.Current opinion in biomedical engineering · 2021Article
- Connecting structure and function from organisms to molecules in small-animal symbioses through chemo-histo-tomography.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Multiple lineages ofeLife · 2021Article
- Spatial metabolomics of in situ host-microbe interactions at the micrometre scale.Nature microbiology · 2020Article
- Imaging mass spectrometry for natural products discovery: a review of ionization methods.Natural product reports · 2020Article
- Common principles and best practices for engineering microbiomes.Nature reviews. Microbiology · 2019Review
- Evolutionary stability of antibiotic protection in a defensive symbiosis.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Variation in Metabolite Profiles of Amphibian Skin Bacterial Communities Across Elevations in the Neotropics.Microbial ecology · 2017Article
- CaMicrobes and environments · 2017Article
- Metabolic fingerprinting of bacteria by fluorescence lifetime imaging microscopy.Scientific reports · 2017Article
- Profiling the metabolic signals involved in chemical communication between microbes using imaging mass spectrometry.FEMS microbiology reviews · 2016Review
- Molecules to Ecosystems: Actinomycete Natural ProductsFrontiers in microbiology · 2016Review
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
One of the greatest challenges in microbial ecology remains to link the metabolic activity of individual cells to their taxonomic identity and localization within environmental samples. Here we combined mass-spectrometric imaging (MSI) through (matrix-assisted) laser desorption ionization time-of-flight MSI ([MA]LDI-TOF/MSI) with fluorescence in situ hybridization (FISH) to monitor antibiotic production in the defensive symbiosis between beewolf wasps and 'Streptomyces philanthi' bacteria. Our results reveal similar distributions of the different symbiont-produced antibiotics across the surface of beewolf cocoons, which colocalize with the producing cell populations. Whereas FISH achieves single-cell resolution, MSI is currently limited to a step size of 20-50 μm in the combined approach because of the destructive effects of high laser intensities that are associated with tighter laser beam focus at higher lateral resolution. However, on the basis of the applicability of (MA)LDI-MSI to a broad range of small molecules, its combination with FISH provides a powerful tool for studying microbial interactions in situ, and further modifications of this technique could allow for linking metabolic profiling to gene expression.
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