Evidence map›Paper›PMID 26171795›Full record

Trial reportThe Journal of clinical endocrinology and metabolism2015

Perilipin 3 Differentially Regulates Skeletal Muscle Lipid Oxidation in Active, Sedentary, and Type 2 Diabetic Males.

Jeffrey D Covington, Robert C Noland, R Caitlin Hebert, Blaine S Masinter, Steven R Smith, Arild C Rustan, Eric Ravussin, Sudip Bajpeyi

Open access · bronzeAbstract readClinical Trial
PubMed Full text at PMC Publisher
In one paragraph

Trial report in The Journal of clinical endocrinology and metabolism, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.

0numbers the graph read from it
0cells of the map it votes in
30citing papers in PubMed
3.4field-weighted citation impact, top 9% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

30 citing papers in PubMed, 40 citations in OpenAlex.

  1. Trial
  2. Article
  3. Article
  4. Article
  5. Article
  6. Article
  7. Perilipins at a glance.Journal of cell science · 2022
    Article
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  10. Article
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  13. Article
  14. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors at 4 institutions in 2 countries.

Jeffrey D CovingtonPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
Robert C NolandPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
R Caitlin HebertPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
Blaine S MasinterPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
Steven R SmithPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
Arild C RustanPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
Eric RavussinPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
Sudip BajpeyiPennington Biomedical Research Center (J.D.C., R.C.N., R.C.H., B.S.M., E.R., S.B.), Laboratory of Skeletal Muscle Physiology, Baton Rouge, Louisiana 70808; Louisiana State University Health Sciences Center (J.D.C.), School of Medicine, New Orleans, Louisiana 70112; Translational Research Institute for Metabolism and Diabetes (S.R.S.), Florida Hospital, Sanford-Burnham Medical Research Institute, Winter Park, Florida 32789; Department of Pharmaceutical Biosciences (A.C.R.), University of Oslo, Oslo, Norway; and Department of Kinesiology (S.B.), University of Texas-El Paso, El Paso, Texas 79968.
Pennington Biomedical Research Center · USSanford Burnham Prebys Medical Discovery Institute · USThe University of Texas at El Paso · USUniversity of Oslo · NO

Funding

Research BaseP30DK072476 · NIDDK · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · PI Leanne Maree Redman · 2005 to 2026
$26.5M
Mentoring Obesity and Diabetes Research in LouisianaP20GM103528 · NIGMS · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · PI GETTYS, THOMAS W · 2012 to 2015
$8.8M
Fat Cell Size, Overfeeding & Ectopic FatR01DK060412 · NIDDK · LSU PENNINGTON BIOMEDICAL RESEARCH CTR · PI RAVUSSIN, ERIC · 2001 to 2011
$5.4M
Skeletal Muscle Mitochondrial Adaptations to a Two-Year Caloric RestictionR01AG030226 · NIA · SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE · PI SMITH, STEVEN RICHARD · 2008 to 2012
$1.6M
NIA NIH HHS NIH1R01AG030226-01A2NIA NIH HHS R01 AG030226NIDDK NIH HHS NIH 1P30 DK072476NIDDK NIH HHS NIH 1P30-DK072476NIDDK NIH HHS NIH R01-DK060412NIDDK NIH HHS P30 DK072476NIDDK NIH HHS R01 DK060412NIGMS NIH HHS NIH P20 GM103528NIGMS NIH HHS NIH P20-GM103528-07NIGMS NIH HHS P20 GM103528
6 · The paper itself

Abstract

contextThe role of perilipin 3 (PLIN3) on lipid oxidation is not fully understood.

objectiveWe aimed to 1) determine whether skeletal muscle PLIN3 protein content is associated with lipid oxidation in humans, 2) understand the role of PLIN3 in lipid oxidation by knocking down PLIN3 protein content in primary human myotubes, and 3) compare PLIN3 content and its role in lipid oxidation in human primary skeletal muscle cultures established from sedentary, healthy lean (leans), type 2 diabetic (T2D), and physically active donors. DESIGN, PARTICIPANTS, AND

interventionThis was a clinical investigation of 29 healthy, normoglycemic males and a cross-sectional study using primary human myotubes from five leans, four T2D, and four active donors. Energy expenditure, whole-body lipid oxidation, PLIN3 protein content in skeletal muscle tissue, and ex vivo muscle palmitate oxidation were measured. Myotubes underwent lipolytic stimulation (palmitate, forskolin, inomycin [PFI] cocktail), treatment with brefeldin A (BFA), and knockdown of PLIN3 using siRNA.

settingExperiments were performed in a Biomedical Research Institute.

main outcome measuresProtein content, 24-hour respiratory quotient (RQ), and ex vivo/in vitro lipid oxidations.

resultsPLIN3 protein content was associated with 24-h RQ (r = -0.44; P = .02) and skeletal muscle-specific ex vivo palmitate oxidation (r = 0.61; P = .02). PLIN3 knockdown showed drastic reductions in lipid oxidation in myotubes from leans. Lipolytic stimulation increased PLIN3 protein in cells from leans over T2Ds with little expression in active participants. Furthermore, treatment with BFA, known to inhibit coatomers that associate with PLIN3, reduced lipid oxidation in cells from lean and T2D, but not in active participants.

conclusionsDifferential expression of PLIN3 and BFA sensitivity may explain differential lipid oxidation efficiency in skeletal muscle among these cohorts.

Indexed as

AdultCarrier ProteinsColforsinCross-Sectional StudiesDiabetes Mellitus, Type 2HumansLipid MetabolismMaleMuscle Fibers, SkeletalMuscle, SkeletalPalmitic AcidPerilipin-1PhosphoproteinsSedentary BehaviorYoung AdultCarrier ProteinsColforsinPalmitic AcidPerilipin-1Phosphoproteins

Identifiers

PMID26171795
PMCPMC4596049
OpenAlexW1849170111

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.