Induction of Apoptosis and Growth Suppression by Homeobox Gene TGIFLX in Prostate Cancer Cell Line Lncap.

Zahra Rashvand, Mansour Heidari, Reza Raoofian, Mohammad Hossein Modarresi, Reza Shirkoohi

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Article in Iranian journal of public health, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

5 authors at 3 institutions in 1 country.

Zahra Rashvand1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.

Mansour Heidari1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran ; 2. Stem Cell Preparation Unit, Farabi Eye Hospital, Tehran University of Medical Sciences , Tehran, Iran.

Reza Raoofian1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.

Mohammad Hossein Modarresi1. Dept. of Medical Genetics, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.

Reza Shirkoohi3. Group of Genetics, Cancer Research Center, Cancer Institute of Iran, Tehran University of Medical Sciences (TUMS) , Tehran, Iran.

Tehran University of Medical Sciences · IRFarabi Hospital · IRMotamed Cancer Institute · IR

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundTGIFLX, a Homoproteins cluster member located on the X chromosome, has a critical role in male reproduction and prostate development. Previous studies have shown the erratic expression of TGIFLX gene in a large proportion of prostate tumors. However TGIFLX function in prostate development remains unknown. The purpose of this study was to evaluate the consequences of TGIFLX expression on prostate cancer cell lines (LNCaP).

methodInducible Tet-On gene expression system was used with a regulatory capability by doxycycline induction. In this system, stable LNCaP cells with TGIFLX tet-on plasmid were able to induce TGIFLX expression by doxycycline treatment. TGIFLX gene expression was confirmed by RT-PCR.

resultsInduction of gene expression caused cell proliferation decrement and apoptosis increment in LNCaP TGIFLX cells compared with control cells (P<0.01). Also, by using PEGFPN1 plasmid protein in this study localization was shown in nucleus. The gene was cloned in the plasmid and transfected to LNcap cells with plasmid PEGFPN1 TGIFLX and the plasmid was PEGFPN1. The TGIFLX expression was confirmed by RT-PCR and fluorescent microscopy.

conclusionTGIFLX expression demonstrated a tumor suppressor characterization in a prostatic cancer cell line with low grade of tumorigenicity (LNCaP). More cell lines with different level of tumorogenicity need to be investigated for further clarification of the TGIFLX gene function.

Indexed as

LNCaPProstate cancerStable cell lineTet-On vectorTGIFLX gene

Identifiers

PMID26171336

PMCPMC4499065

OpenAlexW2107514526

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.