ArticleProceedings of the National Academy of Sciences of the United States of America2015
High-throughput analysis of yeast replicative aging using a microfluidic system.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 97 papers.
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Who cites it
97 citing papers in PubMed, 191 citations in OpenAlex.
- The power of many: when genetics met yeasts and high-throughput.Biological reviews of the Cambridge Philosophical Society · 2026Review
- Article
- BudFinder: A Masked Auto-Encoder vision transformer framework for yeast budding detection and lifespan quantification.PLoS computational biology · 2026Article
- Cdc42 interacts with chaperone Ydj1 to enhance its stability and partitioning during asymmetric cell division and aging in yeast.PLoS biology · 2026Article
- Replicative age differentially predicts metabolic competence across industrial yeasts at single-cell resolution.FEMS yeast research · 2026Article
- Tom70-mediated mitochondria-nuclear envelope contacts regulate nuclear pore complex inheritance during gametogenesis.bioRxiv : the preprint server for biology · 2025Article
- Nucleosome stability safeguards cell identity, stress resilience and healthy aging.Research square · 2025Article
- Article
- Nucleosome stability safeguards cell identity, stress resilience and healthy aging.bioRxiv : the preprint server for biology · 2025Article
- Unveiling the Anti-Aging Potential of 3HB: Lifespan Extension and Cellular Senescence Delay.Nutrients · 2025Article
- Loss of Heterozygosity associated with ubiquitous environments in yeast.PLoS genetics · 2025Article
- Histone H3 cysteine 110 enhances iron metabolism and modulates replicative life span inScience advances · 2025Article
- The core genetic drivers of chronological aging in yeast are universal regulators of longevity.Microbial cell (Graz, Austria) · 2025Article
- A mortality timer based on nucleolar size triggers nucleolar integrity loss and catastrophic genomic instability.Nature aging · 2024Article
- Decoding senescence of aging single cells at the nexus of biomaterials, microfluidics, and spatial omics.npj aging · 2024Review
- The de novo design and synthesis of yeast chromosome XIII facilitates investigations on aging.Nature communications · 2024Article
- Reliable replicative lifespan determination of yeast with a single-channel microfluidic chip.Biology open · 2024Article
- Single-Cell Microfluidics: A Primer for Microbiologists.The journal of physical chemistry. B · 2024Review
- Yeast EndoG prevents genome instability by degrading extranuclear DNA species.Nature communications · 2024Article
- Capturing of extracellular vesicles derived from single cells ofLab on a chip · 2024Article
37 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
Saccharomyces cerevisiae has been an important model for studying the molecular mechanisms of aging in eukaryotic cells. However, the laborious and low-throughput methods of current yeast replicative lifespan assays limit their usefulness as a broad genetic screening platform for research on aging. We address this limitation by developing an efficient, high-throughput microfluidic single-cell analysis chip in combination with high-resolution time-lapse microscopy. This innovative design enables, to our knowledge for the first time, the determination of the yeast replicative lifespan in a high-throughput manner. Morphological and phenotypical changes during aging can also be monitored automatically with a much higher throughput than previous microfluidic designs. We demonstrate highly efficient trapping and retention of mother cells, determination of the replicative lifespan, and tracking of yeast cells throughout their entire lifespan. Using the high-resolution and large-scale data generated from the high-throughput yeast aging analysis (HYAA) chips, we investigated particular longevity-related changes in cell morphology and characteristics, including critical cell size, terminal morphology, and protein subcellular localization. In addition, because of the significantly improved retention rate of yeast mother cell, the HYAA-Chip was capable of demonstrating replicative lifespan extension by calorie restriction.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.