ArticleProceedings of the National Academy of Sciences of the United States of America2015
Navigating natural variation in herbivory-induced secondary metabolism in coyote tobacco populations using MS/MS structural analysis.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- Biosynthetic mechanism and ecological function of chirality in Solanum steroidal alkaloids.Nature communications · 2026Article
- Metabolomic adaptations and genetic polymorphism in ecopopulations ofFrontiers in plant science · 2025Article
- Decoding the biochemical dialogue: metabolomic insights into soybean defense strategies against diverse pathogens.Science China. Life sciences · 2024Article
- A persistent major mutation in canonical jasmonate signaling is embedded in an herbivory-elicited gene network.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- The downside of metabolic diversity: Postingestive rearrangements by specialized insects.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Neutral Loss Mass Spectral Data Enhances Molecular Similarity Analysis in METLIN.Journal of the American Society for Mass Spectrometry · 2022Article
- Metabolization and sequestration of plant specialized metabolites in insect herbivores: Current and emerging approaches.Frontiers in physiology · 2022Review
- How Plants Synthesize Pyrethrins: Safe and Biodegradable Insecticides.Trends in plant science · 2020Review
- The Architecture of Metabolism Maximizes Biosynthetic Diversity in the Largest Class of Fungi.Molecular biology and evolution · 2020Article
- Strigolactone signaling regulates specialized metabolism in tobacco stems and interactions with stem-feeding herbivores.PLoS biology · 2020Article
- Information theory tests critical predictions of plant defense theory for specialized metabolism.Science advances · 2020Article
- Using Gene Expression to Study Specialized Metabolism-A Practical Guide.Frontiers in plant science · 2020Review
- Multi-tissue integration of transcriptomic and specialized metabolite profiling provides tools for assessing the common bean (Phaseolus vulgaris) metabolome.The Plant journal : for cell and molecular biology · 2019Article
- Gastropods and Insects Prefer Different Solanum dulcamara Chemotypes.Journal of chemical ecology · 2019Article
- Article
- Metabolome variability for two Mediterranean sponge species of the genus Haliclona: specificity, time, and space.Metabolomics : Official journal of the Metabolomic Society · 2018Article
- Current Challenges in Plant Eco-Metabolomics.International journal of molecular sciences · 2018Review
- Propagating annotations of molecular networks using in silico fragmentation.PLoS computational biology · 2018Article
- Natural Variation in Elicitation of Defense-Signaling Associates to Field Resistance Against the Spot Blotch Disease in Bread Wheat (Frontiers in plant science · 2018Article
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
Natural variation can be extremely useful in unraveling the determinants of phenotypic trait evolution but has rarely been analyzed with unbiased metabolic profiling to understand how its effects are organized at the level of biochemical pathways. Native populations of Nicotiana attenuata, a wild tobacco species, have been shown to be highly genetically diverse for traits important for their interactions with insects. To resolve the chemodiversity existing in these populations, we developed a metabolomics and computational pipeline to annotate leaf metabolic responses to Manduca sexta herbivory. We selected seeds from 43 accessions of different populations from the southwestern United States--including the well-characterized Utah 30th generation inbred accession--and grew 183 plants in the glasshouse for standardized herbivory elicitation. Metabolic profiles were generated from elicited leaves of each plant using a high-throughput ultra HPLC (UHPLC)-quadrupole TOFMS (qTOFMS) method, processed to systematically infer covariation patterns among biochemically related metabolites, as well as unknown ones, and finally assembled to map natural variation. Navigating this map revealed metabolic branch-specific variations that surprisingly only partly overlapped with jasmonate accumulation polymorphisms and deviated from canonical jasmonate signaling. Fragmentation analysis via indiscriminant tandem mass spectrometry (idMS/MS) was conducted with 10 accessions that spanned a large proportion of the variance found in the complete accession dataset, and compound spectra were computationally assembled into spectral similarity networks. The biological information captured by this networking approach facilitates the mining of the mass spectral data of unknowns with high natural variation, as demonstrated by the annotation of a strongly herbivory-inducible phenolic derivative, and can guide pathway analysis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.