Evidence map›Paper›PMID 26168991›Full record

ArticlePhotosynthesis research2016

Silver island film substrates for ultrasensitive fluorescence detection of (bio)molecules.

Marcin Szalkowski, Khuram U Ashraf, Heiko Lokstein, Sebastian Mackowski, Richard J Cogdell, Dorota Kowalska

Abstract read
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In one paragraph

Article in Photosynthesis research, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
1.2field-weighted citation impact, top 21% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed, 15 citations in OpenAlex.

  1. Article
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors at 2 institutions in 2 countries.

Marcin SzalkowskiFaculty of Physics, Astronomy and Informatics, Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5, 87-100, Torun, Poland.
Khuram U AshrafInstitute of Molecular, Cell & Systems Biology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, G12 8TA, Scotland, UK.
Heiko LoksteinInstitute of Molecular, Cell & Systems Biology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, G12 8TA, Scotland, UK.
Sebastian MackowskiFaculty of Physics, Astronomy and Informatics, Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5, 87-100, Torun, Poland. mackowski@fizyka.umk.pl.
Richard J CogdellInstitute of Molecular, Cell & Systems Biology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, G12 8TA, Scotland, UK.
Dorota KowalskaFaculty of Physics, Astronomy and Informatics, Institute of Physics, Nicolaus Copernicus University, ul. Grudziadzka 5, 87-100, Torun, Poland.
Institute of Physics · PLUniversity of Glasgow · GB

Funding

Biotechnology and Biological Sciences Research Council
6 · The paper itself

Abstract

A silver island film (SIF) substrate was used to demonstrate that Metal-Enhanced Fluorescence (MEF) is a powerful tool to enable detection of emission from (bio)molecules at very low concentrations. The experiments were carried out with the Fenna-Matthews-Olson (FMO) pigment-protein complex from the photosynthetic green sulfur bacterium Chlorobaculum tepidum. FMO was diluted to a level, at which no emission was detectable on a glass substrate. In contrast, the fluorescence of FMO was readily observed on the SIF substrate, even though the emission wavelength of FMO is displaced by over 300 nm from the maximum of the plasmon resonance of the SIF layer. Estimated enhancements of the fluorescence intensity of FMO on SIF are about 40-fold. The enhancement factor correlates with the improvement of the signal-to-noise ratio for FMO emission on SIF substrates.

Indexed as

SilverBacterial ProteinsFluorescenceGlassLight-Harvesting Protein ComplexesMicroscopy, Atomic ForceNanostructuresSignal-To-Noise RatioSpectrometry, FluorescenceBacterial ProteinsFMO bacteriochlorophyll protein, BacteriaLight-Harvesting Protein ComplexesSilverFenna−Matthews−Olson complexFluorescenceMetal-enhanced fluorescencePlasmonSignal-to-noise ratioSilver island film

Identifiers

PMID26168991
OpenAlexW2175931482

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.