ArticleAnalytical and bioanalytical chemistry2015
Determination of porphyrins in oral bacteria by liquid chromatography electrospray ionization tandem mass spectrometry.
Article in Analytical and bioanalytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 65 citations in OpenAlex.
- Comparison of dental plaque reduction after use of electric toothbrushes with and without QLF-D-applied plaque visualization: a 1-week randomized controlled trial.BMC oral health · 2020Trial
- Photoinduced Fluorescence Changes in Selected Bacterial Strains Under 405 nm Laser Excitation: An In Vitro Hue-Based Monitoring Approach.Micromachines · 2026Article
- Colouring dysbiosis: FetB-dependent Mn-PPIX produced by Porphyromonas gingivalis shapes the oral microbiota.NPJ biofilms and microbiomes · 2026Article
- Integrating mass spectrometry and hyperspectral imaging for protoporphyrin IX detection in malignant glioma tissue.Scientific reports · 2025Article
- Autofluorescence properties of wound-associated bacteria cultured under various temperature, salinity, and pH conditions.BMC microbiology · 2025Article
- Photoinactivation by UVA radiation and visible light of Candida auris compared to other fungi.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2024Article
- Innovative Phosphorene Nanoplatform for Light Antimicrobial Therapy.Pharmaceutics · 2023Review
- The influence of growth medium on the photodynamic susceptibility of Aggregatibacter actinomycetemcomitans to antimicrobial blue light.Lasers in medical science · 2023Article
- Fluorescence Spectroscopy Shows Porphyrins Produced by Cultured Oral Bacteria Differ Depending on Composition of Growth Media.Caries research · 2023Article
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- Optical property recovery with spatially-resolved diffuse reflectance at short source-detector separations using a compact fiber-optic probe.Biomedical optics express · 2021Article
- Opto-chemical treatment for enhanced high-level disinfection of mature bacterial biofilm in a Teflon-based endoscope model.Biomedical optics express · 2021Article
- Visible Light-Induced Antibacterial Activity of Pigments Extracted from Dregs of Green and Black Teas.Scientifica · 2021Article
- TheFrontiers in microbiology · 2020Article
- Photoinhibition ofDentistry journal · 2019Article
- Antimicrobial Blue Light Inactivation of Neisseria gonorrhoeae: Roles of Wavelength, Endogenous Photosensitizer, Oxygen, and Reactive Oxygen Species.Lasers in surgery and medicine · 2019Article
- Bacteroides fragilis requires the ferrous-iron transporter FeoAB and the CobN-like proteins BtuS1 and BtuS2 for assimilation of iron released from heme.MicrobiologyOpen · 2019Article
- Dental acid etchant as a sensitizing agent in photodynamic therapy to reduce S. mutans in dentinal carious lesions.Lasers in medical science · 2019Article
- Fluorescence change of Fusobacterium nucleatum due to Porphyromonas gingivalis.Journal of microbiology (Seoul, Korea) · 2018Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Biofilms in the oral cavity can be visualized by fluorescence and a common assumption is that the endogenously produced porphyrins in certain bacteria give rise to this fluorescence. Porphyrin content in oral bacteria has been sparingly investigated, and non-selective detection techniques such as utilizing the Soret fluorescence band of porphyrins are often used. In the present study, a quantitative and selective method for the determination of porphyrins in oral bacteria has been developed and validated using high performance liquid chromatography-tandem mass spectrometry. Lysis of bacteria using Tris-EDTA buffer together with ultrasonication showed high microbial killing efficiency ≥99.98%, and sample clean-up using C18-solid phase extraction resulted in low matrix effects ≤14% for all analytes. Using this method, the porphyrin content was determined in the two oral pathogens Aggregatibacter actinomycetemcomitans and Porphyromonas gingivalis, as well as for baker's yeast, Saccharomyces cerevisiae. Uroporphyrin, 7-carboxylporphyrin, 6-carboxylporphyrin, coproporphyrin, and protoporphyrin IX were identified in the investigated microorganisms, and it was shown that the porphyrin profile differs between the two bacteria, as well as for S. cerevisiae. To our knowledge, this is the first time the porphyrin profile has been determined for the bacterium A. actinomycetemcomitans.
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