ArticleNature communications2015
A highly selective biosynthetic pathway to non-natural C50 carotenoids assembled from moderately selective enzymes.
Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Effects of carotenoid supplementation on liver enzymes in adults: a GRADE-assessed systematic review and dose-response meta-analysis of randomized controlled trials.BMC complementary medicine and therapies · 2025Pooled it
- Comparative Structural Modeling Suggests Distinct Signatures of Conformational Plasticity and Surface Physicochemistry in Phytoene Synthase and Dehydrosqualene Synthase.Molecules (Basel, Switzerland) · 2026Article
- Creating novel metabolic pathways by protein engineering for bioproduction.Trends in biotechnology · 2025Review
- Carotenoids as modulators of the PI3K/Akt/mTOR pathway: innovative strategies in cancer therapy.Medical oncology (Northwood, London, England) · 2024Review
- The Promiscuity of Squalene Synthase-Like Enzyme: Dehydrosqualene Synthase, a Natural Squalene Hyperproducer?Journal of agricultural and food chemistry · 2024Article
- Two Distinct Enzymes Have Both Phytoene Desaturase and 3,4-Desaturase Activities Involved in Carotenoid Biosynthesis by the Extremely Halophilic Archaeon Haloarcula japonica.Microbes and environments · 2024Article
- The Clinical Promise of Microalgae in Rheumatoid Arthritis: From Natural Compounds to Recombinant Therapeutics.Marine drugs · 2023Review
- Microbial production of nutraceuticals: Metabolic engineering interventions in phenolic compounds, poly unsaturated fatty acids and carotenoids synthesis.Journal of food science and technology · 2023Review
- Engineering living and regenerative fungal-bacterial biocomposite structures.Nature materials · 2022Article
- Biosynthetic pathways of triterpenoids and strategies to improve their Biosynthetic Efficiency.Plant growth regulation · 2022Review
- Biochemical and Immunological implications of Lutein and Zeaxanthin.International journal of molecular sciences · 2021Review
- Production of Rainbow Colorants by Metabolically Engineered Escherichia coli.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2021Article
- Pathway Engineering Using Escherichia coli to Produce Commercialized Carotenoids.Advances in experimental medicine and biology · 2021Article
- Protein engineering strategies for microbial production of isoprenoids.Metabolic engineering communications · 2020Review
- Modular engineering for microbial production of carotenoids.Metabolic engineering communications · 2020Review
- Liposome fragment-mediated introduction of multiple plasmids intoBiochemistry and biophysics reports · 2019Article
- Challenges and tackles in metabolic engineering for microbial production of carotenoids.Microbial cell factories · 2019Review
- Genetically engineered biosynthetic pathways for nonnatural CScientific reports · 2019Article
- Discovery and engineering of an endophyticJournal of biological engineering · 2019Article
- Construction of a pathway to C50-ε-carotene.PloS one · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Synthetic biology aspires to construct natural and non-natural pathways to useful compounds. However, pathways that rely on multiple promiscuous enzymes may branch, which might preclude selective production of the target compound. Here, we describe the assembly of a six-enzyme pathway in Escherichia coli for the synthesis of C50-astaxanthin, a non-natural purple carotenoid. We show that by judicious matching of engineered size-selectivity variants of the first two enzymes in the pathway, farnesyl diphosphate synthase (FDS) and carotenoid synthase (CrtM), branching and the production of non-target compounds can be suppressed, enriching the proportion of C50 backbones produced. We then further extend the C50 pathway using evolved or wild-type downstream enzymes. Despite not containing any substrate- or product-specific enzymes, the resulting pathway detectably produces only C50 carotenoids, including ∼ 90% C50-astaxanthin. Using this approach, highly selective pathways can be engineered without developing absolutely specific enzymes.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.